LOCUS       sequence-124640-       12847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of the S. epidermidis Cas10·Csm complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Hatoum-Aslan A, Samai P, Maniv I, Jiang W, Marraffini LA
  TITLE     A ruler protein in a complex for antiviral defense determines the 
            length of small interfering CRISPR RNAs.
  JOURNAL   J Biol Chem. 2013 Sep 27;288(39):27888-97. doi: 
            10.1074/jbc.M113.499244. Epub 2013 Aug 9.
  PUBMED    23935102
REFERENCE   2  (bases 1 to 12847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.499244"; journalName: "J Biol Chem"; date: 
            "2013-09-27- 27"; volume: "288"; issue: "39"; pages: "27888-97"
COMMENT     Sequence Label: pPS22 (pET23a-Cas10/Csm/csm2H6N)
FEATURES             Location/Qualifiers
     source          1..12847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2404..2546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2472..2491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2632..2654
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3348..3367
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3348..3366
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7242..7259
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(12311..12329)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12325..12372
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaattaa tacgactcac
     3361 tatagggaga ccacaacggt ttccctctag aagtgatggc atttgttaaa gtatcggatc
     3421 gatacccacc ccgaagaaaa ggggacgaga acacgtatgc cgaagtatat aaatcatcag
     3481 tacaaaggat cgatacccac cccgaagaaa aggggacgag aactagtaat aattgtcatt
     3541 tgcatacgtt acatcgatga tcgataccca ccccgaagaa aaggggacga gaactagtac
     3601 ggtcgtgaac attttttctt gattctctga tcgatagcca ccccgaagaa aagggggcag
     3661 agtgtaaatc taacaacact ctaaaaaatt gtagattttg aataaaatac gcattgatta
     3721 atttctaggg gatggaaaaa tgaaagatgt tatttatgta gaaaatcatt actttgttac
     3781 tgcgaaggaa aatagcatta aatttagaaa tgtcatagat aaaagtgaaa aattttattt
     3841 gtttgaagaa attgaagcta ttatttttga tcattataaa agctattttt cacataaatt
     3901 ggtaattaaa tgtatagaga atgatattgc gattatattt tgtgacaaaa agcattctcc
     3961 agttacacag ctcatttctt catatggcat ggttaatcgt cttaaaagaa ttcaaagtca
     4021 atttcagtta tcgggaagaa caaaagatag aatttggaaa aagattgtta taaataaaat
     4081 ttttaaccaa acacgatgtc ttgaaaataa tttacataat gaaaatgtta agctgatgtt
     4141 aggttttgca aaagaagtga gttcaggaga caaaagtaat aaagaagcac atgctactcg
     4201 tatttatttt aaagatttat ttggcaaaca atttaaacgt ggacgctata atgatgttat
     4261 caattcagga ttgaattatg gttattcaat tctcagatct tttatcaaca aagagttagc
     4321 tattcatgga tttgaaatga gcctaggtat taaacatcag tcaaaagaaa atccatttaa
     4381 tttagcagat gatatcattg aagtttttcg tccttttgta gataatatag tgtacgagat
     4441 agtttttaag aaaaatattg atacatttga tataaacgaa aagaaattat tattaaatgt
     4501 tttgtatgaa aggtgcatta tagataaaaa agtagtgcga ttacttgata gtgtgaagat
     4561 agttattcaa tcgctaatta gatgttatga agaaaataca cctacttatt tattactacc
     4621 taagatgata gaggtgggaa actaatgtat ttgttagtta gttttgattt acctcgagat
     4681 actaaactag aacgtcgaat tgctagtaaa tatcgtctta gattaataga acttggattt
     4741 agtatgaagc aatttagttt atacgagaga tatgtcagta atgttcaaaa gaaagataaa
     4801 atcttagaaa tattaaagca ggaaatacct gatactggaa gtattacctt atacatattg
     4861 cctgatgaag tgaatagtaa tcagattact atattaggaa aagaagtaaa gcttgctgtc
     4921 aacaaagaac caaaactaat ctttatataa atggaggtta aaatgaataa aaaaaatata
     4981 ttaatgtatg gctctttatt acatgatata gggaaaatta tatatcgaag tggtgatcat
     5041 acattttcaa gaggtacgca ttcaaaatta ggtcatcaat ttttgtccca attttcagaa
     5101 tttaaagaca acgaagtgct tgataacgtt gcttatcatc attacaaaga actcgcaaaa
     5161 gctaatttag ataatgataa tacagcttat attacctata ttgcggataa tattgcgagt
     5221 ggtattgata gaagagatat tatagaagaa ggcgatgaag aatacgaaaa acaactattt
     5281 aattttgata aatatacacc gctatatagt gtgtttaata ttgtgaattc tgaaaaattg
     5341 aaacaaacaa acgggaagtt taaattttct aatgaaagta atattgaata tcctaaaact
     5401 gaaaacattc aatattcaag tggaaattat acaacattaa tgaaagatat gagtcatgat
     5461 ttagagcaca aattaagtat taaagaaggt acatttcctt cattattaca atggacggaa
     5521 agtctatggc aatatgtacc tagttcgaca aataaaaacc aattaattga tatttctctt
     5581 tatgatcata gtcgtattac atgtgccatc gccagttgta tatttgatta tttaaatgaa
     5641 aataacatac ataattacaa agatgaattg ttctcaaagt atgaaaatac caaatcattt
     5701 tatcaaaaag aagctttttt actacttagt atggatatga gtggtattca agattttatt
     5761 tacaatataa gcggttctaa agcattaaag agtctaagat ctcgtagttt ttatttagaa
     5821 ctcatgcttg aagtaatcgt tgatcaatta ttagaaagat tagaattagc acgagcaaat
     5881 cttttgtata caggtggtgg ccatgcttat ttattagtgt ctaatactga taaagtgaag
     5941 aaaaaaataa ctcaatttaa taatgaatta aaaaaatggt ttatgtcaga atttactaca
     6001 gatctttcat tatcaatggc ttttgaaaaa tgtagtggcg atgatttaat gaatacaagt
     6061 ggtaattata gaactatttg gcgtaatgtt agcagcaaac tttctgatat taaagcgcat
     6121 aaatattccg cggaagatat attaaaatta aatcattttc attcgtatgg agatcgggaa
     6181 tgtaaagaat gtttaagaag tgacatagat attaatgatg atggactatg tagtatatgt
     6241 gaaggaatta ttaatatatc aaatgattta agagataaat cattctttgt actgtcagaa
     6301 actggaaaat taaaaatgcc attcaataaa tttatatcgg ttattgatta tgaagaggca
     6361 gaaatgttag tacaaaataa taatcaagtt cgtatttaca gtaaaaataa accatatata
     6421 ggcataggaa tatcaacaaa tttatggatg tgtgattacg actatgctag tcaaaatcaa
     6481 gatatgagag aaaaaggtat tggaagttat gtagatagag aagaaggggt taagcgttta
     6541 ggcgtggtac gtgccgatat agataatctc ggtgctacat ttatatctgg aattccagaa
     6601 aaatataatt caatttcaag aacagctaca ttgtctcgtc aattatcatt attttttaaa
     6661 tacgaattaa atcatttatt agaaaattat caaattactg ctatatattc aggcggtgac
     6721 gatttatttt taatcggtgc atgggatgac attatagaag caagcattta tataaatgac
     6781 aaatttaaag agtttactct tgataaacta acattgtctg ccggggttgg aatgtttagt
     6841 ggtaagtacc cagtttctaa aatggctttt gagacaggac gacttgaaga agcggctaag
     6901 actggtgaaa aaaatcagat atctctttgg ttacaagaaa aagtatataa ctgggatgag
     6961 tttaaaaaga atatcttaga agaaaaactt ctcgttttac aacaggggtt ttctcaaaca
     7021 gatgaacacg ggaaagcctt catttataaa atgctcgctt tactgagaaa taatgaagct
     7081 attaatattg ctcgtttagc ttacttatta gcaagaagca agatgaatga ggattttacg
     7141 tctaaaattt ttaattgggc tcaaaacgac aaagataaaa atcaattaat tacagcgtta
     7201 gagtattata tttatcaaat aagggaggct gattgagtat gcatcaccat caccatcacg
     7261 ctagcatatt agctaaaact aaaagtggta aaacgataga tttgactttt gcacatgagg
     7321 tcgtaaaaag taatgtaaaa aatgttaaag atagaaaagg caaagaaaaa caagttttat
     7381 ttaacgggct tacaacaagc aagttaagaa atttaatgga gcaggtaaat agactttata
     7441 ctattgcatt taattcgaat gaggatcaat tgaatgaaga attcattgat gaattagaat
     7501 atttaaaaat taaattttat tatgaagcag gacgagaaaa aagcgttgat gaatttttaa
     7561 aaaaaacatt gatgtttcca attattgata gagtgataaa aaaagaatca aaaaaatttt
     7621 tcttagatta ttgtaaatac tttgaagctt tagttgcata cgctaaatat tatcaaaagg
     7681 aggattaata tgtattcaaa aattaaaatt tcaggaacaa ttgaagtagt tactggttta
     7741 cacatcggcg gaggcggtga atctagtatg attggagcaa ttgattctcc tgtagttaga
     7801 gatttgcaaa ccaaattacc tatcatacct ggcagttcaa tcaaaggaaa aatgagaaat
     7861 ttattagcaa aacattttgg cttgaaaatg aaacaagaga gtcataacca agatgatgaa
     7921 cgtgtattaa gattatttgg ctcaagtgaa aaaggaaata tccaaagagc tcgtctacaa
     7981 atttctgatg cattcttttc tgaaaagaca aaagagcatt ttgcgcaaaa tgatattgcc
     8041 tatacagaaa cgaaatttga gaatacaatt aatcgtttaa ctgcagttgc aaacccaaga
     8101 caaattgaaa gagtaacaag aggatctgag tttgactttg tatttattta caatgtcgat
     8161 gaagagtcgc aagttgagga tgattttgag aatattgaaa aagcgattca cttattagag
     8221 aatgactatc ttggtggcgg cggaactaga ggtaacggac gtattcaatt taaagataca
     8281 aatatcgaga cagttgttgg agaatacgat agtacaaatc ttaaaattaa gtaggtgaat
     8341 gacattggca acaaaagtat ttaaactttc ttttaagact cctgttcatt ttggaaaaaa
     8401 acggttgtca gatggtgaaa tgacaatcac tgctgatact ttgtttagtg ctttatttat
     8461 tgaaacactt caattgggta aagataccga ttggttatta aatgatttaa tcattagtga
     8521 tacatttcct tatgagaatg agctttatta tcttcctaaa cctttgataa aaattgactc
     8581 taaagaagaa gataaccata aagcatttaa aaagttaaaa tatgttccgg ttcatcacta
     8641 taatcaatat ttaaatggag agttaagcgc tgaagatgcg acagatttaa atgatatttt
     8701 taatattggg tatttttctc tacaaacaaa ggtttcatta atagcacaag aaactgattc
     8761 aagtgctgat agtgaacctt attcagtggg aacatttact tttgaacctg aagcgggttt
     8821 atattttatt gcaaaaggat cagaagaaac gcttgaccat ttaaataata ttatgactgc
     8881 attacagtat tcaggtttag gtggtaaacg taatgcagga tacggacaat ttgaatatga
     8941 aataataaat aatcaacaac tatctaagtt actgaatcaa aatggaaaac attctattct
     9001 tttatcaacg gcaatggcta aaaaagaaga gatagagagt gctttaaaag aggcgagata
     9061 cattttaact aaacgttctg gtttcgtaca atcaacgaat tattctgaaa tgctagttaa
     9121 aaaaagtgat ttctatagct tttcttcggg ttcagttttt aaaaatatct ttaatggtga
     9181 tatttttaat gttggccata atggtaaaca cccagtctat cgctatgcaa aacctttatg
     9241 gttggaggta taagtatgac aataaaaaat tatgaagtcg ttattaaaac tttaggtcca
     9301 attcatattg gtagtggtca agttatgaag aagcaagatt acatttatga cttttataat
     9361 tctaaagttt atatgattaa tggaaataaa ctagttaaat ttttaaaaag aaaaaattta
     9421 ctttatacat atcaaaactt tttgaggtac ccaccaaaaa atccaagaga aaatggactt
     9481 aaagactatt tagacgctca aaatgttaag caaagtgaat gggaagcatt tgtgagttat
     9541 tctgaaaagg tcaatcaagg taagaaatat ggtaacacac gtcctaaacc gctaaatgat
     9601 ttacacttaa tggtaagaga cggtcaaaat aaagtgtatc ttccaggtag ttcaatcaaa
     9661 ggtgctatca aaacaactct cgtgtcaaaa tataataatg aaaaaaacaa agacatttat
     9721 agcaaaatta aagtcagcga ttcaaaacct attgatgaaa gtaatttagc gatttatcaa
     9781 aaaatagaca ttaataaaag tgaaaaatca atgcctttat atagagagtg catagatgta
     9841 aataccgaaa taaaatttaa gttaacaatt gaagatgaaa tttattctat taatgaaatt
     9901 gaacaaagta tccaagattt ttacaaaaac tattatgata aatggttagt cggattcaaa
     9961 gaaacaaaag gtggaagacg atttgcatta gagggcggta taccagatgt cctaaatcag
    10021 aatattttgt tcttaggtgc tgggacagga tttgttagta aaacaacaca ctatcaatta
    10081 aaaaatcgaa aacaagctaa acaagattct tttgagattt taactaaaaa attccgagga
    10141 acttatggga aaatgaagga aataccttct aacgtaccag ttgctttaaa aggaacaact
    10201 aatcaaagtc gtcatacttc atatcagcaa ggaatgtgta aagtgagttt tcaagagtta
    10261 aataatgagg tgctataatg aaaatattat ttagtccaat aggtaattca gatccatgga
    10321 gaaatgatag agatggtgcg atgcttcata tcgtgcgtca ttataattta gataaggttg
    10381 tattatattt tactagaact atttgggaag gaaatgaaaa tagaaaaggc cataaaattt
    10441 acgaatggga aaaaattatc caaactgttt ctccaaatac tgaagtagaa atcattattg
    10501 aaaatgtaga caatgctcaa gattatgatg tcttcaagga gaaatttcat aagtatttaa
    10561 aaataattga agatagctat gaagattgtg aaataatttt gaatgtcact agtggtacac
    10621 cccaaatgga atcaacatta tgtttagaat atatcgtcta tcctgaaaat aagaagtgtg
    10681 tacaagtgag cacgccaact aaggatagta atgcaggtat cgaatattca aatcctaaag
    10741 ataaagtaga agaatttgaa atagtcaatg aagtagaaaa gaaatctgaa aaacgttgta
    10801 aagaaataaa cattttaagc tttagagaag caatgattag atctcaaatt ctcggtttaa
    10861 tagataatta tgattatgaa ggtgctctta atttagtaag taatcaaaaa tcttttcgca
    10921 atgggaaatt attaagaaaa aaactactat cattaacaaa acaaattaaa acacatgaag
    10981 tttttccaga aattaatgag aagtacagag atgatgcttt aaaaaaatca ctatttcatt
    11041 atttactgtt aaatatgaga tataatcgtc ttgatgtagc tgaaacgtta attagagtaa
    11101 aatctattgc tgagtttata cttaaaacat acattgagat tcattggcct actttaataa
    11161 ttgaaaaaga tggtaaacct tatctaaatg atgaagataa tttatctttt gtttataaat
    11221 ataatctgtt attagaaaaa agaaaacaaa attttgatgt ttcaagaatt ttgggacttc
    11281 ctgcattcat tgatatactc acaattttag aacctaattc tcaactatta aaagaagtca
    11341 atgcagtaaa cgatataaat ggtttaagaa attccatagc ccataattta gatacattaa
    11401 atttggataa aaataaaaat tataagaaaa taatgttatc tgttgaagcg ataaagaata
    11461 tgttacacat ctcatttcct gagatagagg aagaagacta taattatttt gaagaaaaaa
    11521 ataaggaatt taaagagcta ttatgataaa taaaattaca gtagagttag acttgccaga
    11581 aagtattcgg tttcaatatt taggaagtgt tttacatggt gtgttaatgg attatctatc
    11641 tgatgatatt gctgaccaat tacatcatga atttgcttat agcccattga aacaaagaat
    11701 atatcataaa aataaaaaaa tcatttggga aattgtatgt atgtcagata atttatttaa
    11761 agaggttgtt aaactattta gttctaaaaa tagtttgctt ttgaaatatt atcaaacaaa
    11821 tattgacatt caatcatttc aaattgagaa gataaatgtt cagaacatga tgaaccaact
    11881 gttacaagta gaagatctaa gtcgttatgt acgtcttaat atacaaacac ctatgtcttt
    11941 taaatatcag aacagttaca tgatttttcc tgatgttaaa cgttttttta gaagtattat
    12001 gatacaattt gacgcgtttt ttgaagaata tagaatgtac gacaaagaaa cattaaattt
    12061 tctagaaaag aatgttaata ttgttgacta caaattgaaa agtacacgtt ttaacttgga
    12121 aaaagttaaa attccttcat ttacaggaga aatagtattt aaaattaaag gacccttacc
    12181 ttttctacag ttaactcatt ttttattaaa gtttggcgaa ttttcaggtt caggtataaa
    12241 aacaagctta ggtatgggaa aatatagtat aatttaatta agacaaagga agctgagttg
    12301 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
    12361 aggggttttt tgctgaaagg aggaactata tccggatcrn srtrwardcc acaacggttt
    12421 ccctctagaa gtgatggcat ttgttaaagt atcrvrscag cagccaactc agcttccttt
    12481 gtcttaatta aattatacta tattttcccr vctrrwardg gaaaatatag tataatttaa
    12541 ttaagacaaa ggaagctgag ttggctgctg rvrsgatact ttaacaaatg ccatcacttc
    12601 tagagggaaa ccgttgtggs crnsrtwdcc acaacggttt ccctctagaa gtgatggcat
    12661 ttgttaaagt atcscrnsrt rvcagcagcc aactcagctt cctttgtctt aattaaatta
    12721 tactatattt tccscrvctr wdggaaaata tagtataatt taattaagac aaaggaagct
    12781 gagttggctg ctgscrvctr rvgatacttt aacaaatgcc atcacttcta gagggaaacc
    12841 gttgtgg
//
