LOCUS       sequence-124642-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression in mammalian cells of an ""INT"" 
            construct_bearing P4_P6 (internally appended with three PP7 
            stem-loops), targeting the GLuc reporter. U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_INT[P4-P6(3xPP7)]
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(472..491)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(655..672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(655..671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(664..686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(880..899)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     999..1021
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgcaggttta aacagtcgac tctagactta attaagaaaa aaagcaccga ctcggtgcca
       61 ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgcg aatacgagag
      121 atgaactgca tccatatcaa cagagaccag cgacgccata tcgtctgctg gagaccacgg
      181 agcccatatg ctctcgtgga gaggagcgac gccatatcgt ctgctccaga ctgttgactt
      241 aggacttggc tgcgtgttag gaccatgtcc gtcagcttat taccataccc tttgcaaggc
      301 catctcaaag tttcccctga gacttggtac tgaacggctg ttgacccctt tcccgcaatt
      361 ccagactcgt attcgcagca tagctcttaa acatagtgag tcgtatctag atcggtgttt
      421 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
      481 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
      541 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt
      601 atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaacactggc
      661 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      721 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      781 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      841 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
      901 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      961 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1021 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcct
//
