LOCUS       sequence-124676-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' 
            Box expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/3' Box_(GLuc)_INT
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(391..407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(404..426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    415..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(439..445,446..463,464..469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(622..639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(873..892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggcg atctagatac gactcactat
       61 gttttagagc tacgaatacg agggagcaga cgatatggcg tcgctcctct ccacgagagc
      121 atatgggctc cgtggtctgc agcagacgat atggcgtcgc tgctctcgta gatgccatat
      181 ggggcactac gtctccagca gacgatatgg cgtcgctggc tcgtattcgt agcaagttaa
      241 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttactttctg
      301 gagtttcaaa agtagactgt acgctaaggg tcatatcttt ttttgtttgg tttgtgtctt
      361 ggttggcgtc ttaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      421 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      481 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      541 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      901 ctccctcgtg cgctctcctg ttccgaccct gc
//
