LOCUS       sequence-124692-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-OUT A3 variant - antisense (UTR sequence from Mutalik et al., 
            Nat. Chem. Biol., 2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAOUTA3
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(386..429)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(653..670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(904..923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      421 gatgatttct ggaattctaa agatctttga cagctagctc agtcctaggt ataatgaatt
      481 ctcgcacatc ttgttgtctg attattgatt tttcccgaaa ccatttgatc atatgacaag
      541 atgtgtatgc ctctacctgc ttcggccgat aaagccgacg ataatactcc caaagcccgc
      601 cgaaaggcgg gctttttttt ggatccttac tcgagtctag actgcaggct tcctcgctca
      661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
      841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
//
