LOCUS       sequence-124724-       10748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. Cre-removable and tet/dox controllable 
            expression of FLAG-HA-tagged transgene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 10748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVE-FH-IN
FEATURES             Location/Qualifiers
     source          1..10748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3488..3497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3497..3520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3521..3547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3606..4180,4181..4183,4184..4192)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3773..3790)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4000..4019
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4193..4987
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4247..4266)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4857..4876
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5310..5343)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5363..5543
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5568..5586)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5569..5586)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5876..5895)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6109..6128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6228..6250
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6288..6306)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6374..6478
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6479..6547,6548..7339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6697..6716)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7510..8098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7999..8018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8252..8269
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8339..8359)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8344..8673
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8524..8659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9807..9872
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10002..10022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10447..10581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10484..10503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10538..10557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgagggcc accatggact acaaggacga cgatgacaag tacccttatg acgtgcccga
     3541 ttacgcttct ggtggcggtg gctcgggcgg aggtgggtcg ggtggcggcg gatcaggatc
     3601 ctaaccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     3661 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     3721 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     3781 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     3841 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     3901 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3961 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     4021 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4081 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     4141 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgattga
     4201 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4261 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4321 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4381 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4441 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4501 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4561 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4621 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4681 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     4741 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4801 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4861 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4921 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4981 cttctgagaa ttcatattac ccctaacacc tgccacccca gtcttaatca gtggtggaag
     5041 aacggtctca gaactgtttg tctcaattgg ccatttaagt ttaatagtaa aagactggtt
     5101 aatgataaca atgcatcgta aaaccttcag aaggaaagaa tgttgtggac catttttttt
     5161 gtgtgtggca gttttaagtt attggtacct ttaagaccaa tgacttacaa ggcagctgta
     5221 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5281 agacaagatg ggatcaattc accatgggaa taacttcgta tagcatacat tatacgaagt
     5341 tatgctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5401 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5461 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5521 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     5581 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     5641 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     5701 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     5761 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     5821 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     5881 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     5941 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     6001 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     6061 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     6121 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     6181 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     6241 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     6301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6721 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7021 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7621 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     7921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8281 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     8341 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8401 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8461 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8521 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8581 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8641 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     8701 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     8761 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     8821 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     8881 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     8941 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     9001 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     9061 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     9121 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     9181 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     9241 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     9301 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     9361 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     9421 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     9481 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     9541 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     9601 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     9661 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     9721 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     9781 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     9841 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     9901 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     9961 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
    10021 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
    10081 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
    10141 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
    10201 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
    10261 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
    10321 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
    10381 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10441 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10501 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10561 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    10621 cccaccccat accctattac cactgccaat tacctagtgg tttcatttac tctaaacctg
    10681 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10741 cttagtag
//
