LOCUS       sequence-124768-         410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4
FEATURES             Location/Qualifiers
     source          1..410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagtactcca ttgggctcga tatcggtacc aacagcgtcg gctgggccgt cattacggac
       61 gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata
      121 aagaagaacc tcattggagc cctcctgttc gactccgggg agacggccga agccacgcgg
      181 ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag
      241 gagatcttta gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag
      301 tcctttttgg tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg
      361 gacgaggtgg cgtaccatga aaagtaccca accatatatc atctgaggaa
//
