LOCUS       sequence-124819-         481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2
FEATURES             Location/Qualifiers
     source          1..481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(389..415)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(428..454)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 attctagagg ctcgagtcat tacccggggt ctttctgacg gaggtggatc ttggtgtgcc
       61 gtgtcaggtc gtcgctccgc gcgaacttcc tgccgcagat gtcacaggcg aagggctttt
      121 ctcctgtgtg ggtgcggatg tgccgggtca ggtggccctt gtcgctgaag ttcctcatgc
      181 agatgcggca ttgaaacggt ttttgccctg tgtgaatgcg gatgtgctgt gtcaggtggt
      241 cgctccgact gaagcggcgg tcacaggact cgacaggaca cgcgtatggc ttctcaccag
      301 tgtgcgtgcg ttggtgccgt gtcaggtggc tcttctccga gaaggacttg ccacattcag
      361 gacatttata gggctttccg gattcgaaat ccagtttctt tttcttagct gccggagcgg
      421 cggatccagc gtaatctgga acgtcataag gatacgatcc tgcatagtcc gggacgtcat
      481 a
//
