LOCUS       sequence-124849-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF10-TF with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-129
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..168)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(448..468)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(469..492)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctagcttaa caattaatta acatatcgag atcgaaatcg tccagagcat cggagccgag
       61 catgtccaga tcaaagtcat ccaatgcgtc gcttcccaac atatccaggt caaagtcatc
      121 gagggcatca gaacccagca tgtcgagatc gaaatcgtcc agcgcgtcgg cgcgcccgga
      181 tcctctcagg tgggttttta ggtgtctagt caaatttgga tgttgactga agttgcgcat
      241 gcatattcgg cattggaatg gcttctcgcc ggtgtgcgta cgtagatgtc tagtcaaatt
      301 accaccttgg gagaaatttc gcatacagat ccgacactga aacggttttt caccggtatg
      361 agtacgggta tgtctagcca aagcttgagc agacgaaaag ttccgcatgc aaatgcgaca
      421 ctggaagggg cgctccccgg gtctagagac cttgcgcttt ttcttgggct tgtcatcgtc
      481 gtccttgtag tccatgatat caccggttgc tttgaattag cggtggtttt cacaacacct
      541 aaaaaagggt ttaaaagata cctttgaacc gctaagaagc ccgagaatta gctccgctca
      601 aaactcaagg ggacaaattc caaaaatgac ttccagcgcc aggctggcct gactagtctc
      661 cacccaccaa atgtgaacaa actccaacgc cattacatcc cctccccccg ccgcgactag
      721 ccgtgctcaa aagcccgagg tgactattgc ggccgatagg accacggggt cacag
//
