LOCUS       sequence-124875-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF5 Op -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-135
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    142..233
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(174..193)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    247..295
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttac ccctacaccg ctagcgagct
      121 caggtaccgg ccagttaggc cagagaaatg ttctggcacc tgcacttgca ctggggacag
      181 cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta
      241 tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattgcggc
      301 ctgtccaata cctcccgtac cttaatatta cttacttatc cttgagagac gtactagtaa
      361 ccctgataaa tgcttcaata atattgaaaa aggacgagta tgagcatcca acattttcgt
      421 gtcgcactca ttcccttctt tgcggcattt tgcttgcctg tttttgcaca ccccgaaacg
      481 ctggtgaaag taaaagatgc tgaagatcaa ctgggtgcaa gagtgggcta tatcgaactg
      541 gatctcaata gcggcaagat ccttgagtct ttccgccccg aagaacgatt cccgatgatg
      601 agcactttca aagtactgct atgtggcgcg gtgttgtccc gtatagacgc cgggcaagag
      661 cagcttggtc gccgtataca ctactcacaa aacgacttgg ttgagtactc gccggtcacg
      721 gaaaagcatc ttacggatgg catgacggta agagaattgt gtagtgctgc cattaccatg
      781 agcgacaaca ccgcggccaa cttacttctg acaacgatcg gaggccctaa ggagctgact
      841 gcatttcttc ataatatggg tgatcatgtg acccggcttg accgctggg
//
