LOCUS       sequence-124879-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(402..428)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(441..530)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(531..551)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acgactcact atagttctag aggctcgagt cattacccgg ggtctttctg acggaggtgg
       61 atcttggtgt gctgtgtcag ggtgctccgg tccgcgaact tcctgccgca gatgtcacag
      121 gcgaagggct tttctcctgt gtgggtgcgg atgtgccgtg tcaggtggtg ggcacagctg
      181 aagttcctca tgcagatgcg gcattgaaac ggtttttgcc ctgtgtgaat gcggatgtgc
      241 cgtgtcaggt ggctcttctc actgaagcgg cggtcacagg actcgacagg acacgcgtat
      301 ggcttctcac cagtgtgcgt acgttggtgc cgtgtcaggt ctccgctctg cgagaaggac
      361 ttgccacatt caggacattt atagggcttt ccggattcga aatccagttt ctttttctta
      421 gctgccggag cggcggatcc agcgtaatct ggaacgtcat aaggatacga tcctgcatag
      481 tccgggacgt catagggata gcccgcatag tcaggaacat cgtatgggta taccttcctc
      541 ttcttcttgg gcccgccggt gccgtctcca ccgagctgag agaggtcgat tcttgtttca
      601 tagagccccg taattgactg atgaatcagt gtg
//
