LOCUS       sequence-124891-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF8 Op -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-139
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(49..80)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    136..227
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(168..187)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    241..289
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgcccttgct caccatggtg gctttaccaa cagtaccgga ttgccaagct ggaagtcgag
       61 cttccattat ataccctcta gtgtctaagc ttggaccact tcggctagcg agctcaggta
      121 ccggccagtt aggccagaga aatgttctgg cacctgcact tgcactgggg acagcctatt
      181 ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt
      241 cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg cggcctgtcc
      301 aatacctccc gtaccttaat attacttact tatccttgag agacgtacta gtaaccctga
      361 taaatgcttc aataatattg aaaaaggacg agtatgagca tccaacattt tcgtgtcgca
      421 ctcattccct tctttgcggc attttgcttg cctgtttttg cacaccccga aacgctggtg
      481 aaagtaaaag atgctgaaga tcaactgggt gcaagagtgg gctatatcga actggatctc
      541 aatagcggca agatccttga gtctttccgc cccgaagaac gattcccgat gatgagcact
      601 ttcaaagtac tgctatgtgg cgcggtgttg tcccgtatag acgccgggca agagcagctt
      661 ggtcgccgta tacactactc acaaaacgac ttggttgagt actcgccggt cacggaaaag
      721 catcttacgg atggcatgac ggtaagagaa ttgtgtagtg ctgccattac catgagcgac
      781 aacaccgcgg ccaacttact tctgacaacg atcggaggcc ctaaggagct gactgcattt
      841 cttcataata tgggtgatca tgtgaccc
//
