LOCUS       sequence-124912-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF7-ZF8 Op 2gap -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-165
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    153..244
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(185..204)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    258..306
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttgg accacttcga ccctccagct
      121 gctagcgagc tcaggtaccg gccagttagg ccagagaaat gttctggcac ctgcacttgc
      181 actggggaca gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta
      241 tgttagtact atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat
      301 tttattgcgg cctgtccaat acctcccgta ccttaatatt acttacttat ccttgagaga
      361 cgtactagta accctgataa atgcttcaat aatattgaaa aaggacgagt atgagcatcc
      421 aacattttcg tgtcgcactc attcccttct ttgcggcatt ttgcttgcct gtttttgcac
      481 accccgaaac gctggtgaaa gtaaaagatg ctgaagatca actgggtgca agagtgggct
      541 atatcgaact ggatctcaat agcggcaaga tccttgagtc tttccgcccc gaagaacgat
      601 tcccgatgat gagcactttc aaagtactgc tatgtggcgc ggtgttgtcc cgtatagacg
      661 ccgggcaaga gcagcttggt cgccgtatac actactcaca aaacgacttg gttgagtact
      721 cgccggtcac ggaaaagcat cttacggatg gcatgacggt aagagaattg tgtagtgctg
      781 ccattaccat gagcgacaac accgcggcca acttacttct gacaacgatc gga
//
