LOCUS       sequence-124915-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZF9 Op x2 -- GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-190
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(55..86)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    156..247
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(188..207)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    261..309
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggattgc caagctggaa
       61 gtcgagcttc cattatatac cctctagtgt ctaagcttct ccatctacat ttctccatct
      121 acagctagcg agctcaggta ccggccagtt aggccagaga aatgttctgg cacctgcact
      181 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
      241 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
      301 tattttattg cggcctgtcc aatacctccc gtaccttaat attacttact tatccttgag
      361 agacgtacta gtaaccctga taaatgcttc aataatattg aaaaaggacg agtatgagca
      421 tccaacattt tcgtgtcgca ctcattccct tctttgcggc attttgcttg cctgtttttg
      481 cacaccccga aacgctggtg aaagtaaaag atgctgaaga tcaactgggt gcaagagtgg
      541 gctatatcga actggatctc aatagcggca agatccttga gtctttccgc cccgaagaac
      601 gattcccgat gatgagcact ttcaaagtac tgctatgtgg cgcggtgttg tcccgtatag
      661 acgccgggca agagcagctt ggtcgccgta tacactactc acaaaacgac ttggttgagt
      721 actcgccggt cacggaaaag catcttacgg atggcatgac ggtaagagaa ttgtgtagtg
      781 ctgccattac catgagcgac aacaccgcgg ccaacttact tctgacaacg atcggaggcc
      841 ctaaggagct gactgcattt cttcataata tgggtgatca tgtgacccgg cttgaccgct
//
