LOCUS       sequence-124933-         472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALE for mouse major satellite with VP64 and FLAG under 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Ishiuchi T, Enriquez-Gasca R, Mizutani E, Boskovic A, 
            Ziegler-Birling C, Rodriguez-Terrones D, Wakayama T, Vaquerizas JM, 
            Torres-Padilla ME
  TITLE     Early embryonic-like cells are induced by downregulating 
            replication-dependent chromatin assembly.
  JOURNAL   Nat Struct Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3066.
  PUBMED    26237512
REFERENCE   2  (bases 1 to 472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2015 Aug 3. doi: 10.1038/nsmb.3066."
COMMENT     Sequence Label: pTAL-MajSat-FLAG-NLS-VP64
FEATURES             Location/Qualifiers
     source          1..472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             115..135
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             160..309
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc
       61 acgtcccatc gcgttgcggg atccatggac tacaaggacg acgatgacaa gagccccaag
      121 aagaagagaa aggtggaggc cagcggttcc ggacgggctg acgcattgga cgattttgat
      181 ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat
      241 gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg
      301 gacatgctga ttaactctag ttgagaattc tgtacatatg ggcaccaaag aacctgtaaa
      361 cgttatcttt ttaaattgaa tgtgcacaaa taaaagtttg gaaaagaaaa aaaaaaaaaa
      421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//
