LOCUS       sequence-124967-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter with wild-type qacR on single plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES005 (pTet-qacR wt/pQacA-deGFP)
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     291..310
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     550..571
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             608..630
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
       61 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
      121 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
      181 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      241 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      301 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      361 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
      421 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      481 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      541 ggccgtttac gtcgccgtcc agctcgacca ggatgggaac aacgccagtg aaaagctcca
      601 tggtatatct ccttcttaaa gttaaacaaa attattgctt ttaagttccc tccaatcctt
      661 atagaccgtg cgatcggtct ataaggatta taatctattt ttttacatat tacaacaaaa
      721 aatcttgcga aatttaaatg
//
