LOCUS       sequence-124976-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces
            ~10-fold higher titer virus. 3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiCRISPR v2
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     133..152
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
       61 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      121 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      181 ttatatatct tgtggaaagg acgaaacacc ggagacggtt gtaaatgagc acacaaaata
      241 cacatgctaa aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa
      301 taactttagt atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat
      361 gagcaaaata aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat
      421 agctactcca ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc
      481 ttcaaatgtt ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt
      541 cattagttta acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac
      601 aaattctaat ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa
      661 taaacaattt tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt
      721 catgtcttct aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat
      781 atcaatttct gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt
      841 tgtgcatgca cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa
      901 taattcttta caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc
      961 aattgc
//
