LOCUS       sequence-124980-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj12-o11
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    252..270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             384..395
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             411..1121
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    1128..1148
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agacagaaaa gcccgccttt cggcgggctt tgcccagaat
       61 cccttcgagc aaatcctagc gaggtgccgc tagattcgct caaaggaacc cttcttcctg
      121 cccgtctgga atccaggaca cctgccaagg gcctttcggc tggtatgtat ttcgtcctat
      181 ttgggactca tcagctggat gtacgaaggc agcgccgtgc tcagtatctc tatcactgat
      241 agggatgtca atctctatca ctgataggga gctagccaat tgatgcatgc tagcataaat
      301 gtgagcggat aacattgaca ttgtgagcgg ataacaagat actgagcacc ctcgcataat
      361 ttcacttctt caatcctccc gttaaagagg agaaattatg aatgactagc cgtaaaggcg
      421 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      481 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      541 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
      601 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
      661 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
      721 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
      781 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
      841 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
      901 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
      961 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1021 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1081 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgatgatac tagtagcggc
     1141 cgctgcag
//
