LOCUS       sequence-124994-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C Trans V1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-255
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..164)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(277..288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcttaacaat taattaacat atcgagatcg aaatcgtcca gagcatcgga gccgagcatg
       61 tccagatcaa agtcatccaa tgcgtcgctt cccaacatat ccaggtcaaa gtcatcgagg
      121 gcatcagaac ccagcatgtc gagatcgaaa tcgtccagcg cgtcggcgcg ccctccgctg
      181 ccgttgcctc tctcgccgca attatggacg acaacctggt tggcaagcaa aaactgatga
      241 tcagaatcat cagataaagt aatcccataa taatcgtctt ccttcaattc ttgtaactcg
      301 aaataaaatc cgcggcactc acgtgcaaaa gcagcggcgg gagcaggcct gaatttttta
      361 gagccggcac acttcgaaag aacgttaagc aaaacggatc cgcctccgga cccgccacca
      421 gatccacctc cggacccacc tcctgagcct ccacctgatc cgcccccaga tcccctcagg
      481 tgggttttca ggtggcgttt gaggctggcg cccacggaga agttgcgcat gcagatccga
      541 cactgaaatg gtttttctcc agtgtgtgtt ctaaggtgat ttgtaagatg tggcctctgg
      601 ctaaaatttc gcatacagat gcggcactgg aagggttttt cgccggtgtg ggtgcgggta
      661 tggcggtgca gatgatcttt cttagaaaag tttctcatgc agattctgca ctggaaaggt
      721 ctctccccgt ccttctgcct cagatgagtc ttaaggtgtc tatccaagtt cactgtctgg
      781 ctaaagttgc gcatgcaaat tcgacactga aatggttttt cgccggtatg
//
