LOCUS       sequence-125003-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  8x Target 1 0gap minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-309
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    480..501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(516..521,522..539,540..546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    554..570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     559..581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     578..594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     578..594
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcctctcccg cgcggttggc cgattcatta atgcagcgga tctctcgagt taatttcgct
       61 taatttcgcg gatccggacc acttcccctc cagcacctcc tgcagcttct tcaagatccg
      121 gaccacttcc cctccagcac ctcctgcagc ttcttcaaga tccggaccac ttcccctcca
      181 gcacctcctg cagcttcttc aagatccgga ccacttcccc tccagcacct cctgcagctt
      241 cttcaagatc cggaccactt cccctccagc acctcctgca gcttcttcaa gatccggacc
      301 acttcccctc cagcacctcc tgcagcttct tcaagatccg gaccacttcc cctccagcac
      361 ctcctgcagc ttcttcaaga tccggaccac ttcccctcca gcacctcctg cagcttcttc
      421 aagatccgct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      481 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      541 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      601 tacgccaagc ttgggctgca ggtcgaggga tctccataag agaagaggga cagctatgac
      661 tgggagtagt caggagagga gg
//
