LOCUS       sequence-125007-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  8x Target 1 4gap minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-310
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    502..523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(538..543,544..561,562..568)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    576..592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     581..603
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     600..616
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct taatttcgcg
       61 gatccggacc acttcccctc cagctgacac ctcctgcagc ttcttcaaga tccggaccac
      121 ttcccctcca gctgacacct cctgcagctt cttcaagatc cggaccactt cccctccagc
      181 tgacacctcc tgcagcttct tcaagatccg gaccacttcc cctccagctg acacctcctg
      241 cagcttcttc aagatccgga ccacttcccc tccagctgac acctcctgca gcttcttcaa
      301 gatccggacc acttcccctc cagctgacac ctcctgcagc ttcttcaaga tccggaccac
      361 ttcccctcca gctgacacct cctgcagctt cttcaagatc cggaccactt cccctccagc
      421 tgacacctcc tgcagcttct tcaagatccg ctggcacgac aggtttcccg actggaaagc
      481 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      541 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      601 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
      661 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
      721 aaacatgtaa ggaaaatttt agggatgtta aag
//
