LOCUS       sequence-125014-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Suicide plasmid for efficient gene mutation in Gram-positive 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdelhamed H, Lawrence ML, Karsi A
  TITLE     A novel suicide plasmid for efficient gene mutation in Listeria 
            monocytogenes.
  JOURNAL   Plasmid. 2015 May 31;81:1-8. doi: 10.1016/j.plasmid.2015.05.003.
  PUBMED    26038185
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2015-05-31"; volume: "81:1-8. doi"; pages: " 
            10.1016/j.plasmid.2015.05.003"
COMMENT     Sequence Label: pHoss1
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..46
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    113..131
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             153..779
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRRHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(229..248)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tattcccatc aagcttattt taattatact ctatcaatga tagagtgtca atattttttt
       61 tagtttttca tgaactcgag gggatccaaa taaaaaacta gtttgacaaa taactctatc
      121 aatgatagag tgtcaacaaa aaggaggaat taatgatgtc tagattagat aaaagtaaag
      181 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
      241 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
      301 gggctttgct cgacgcctta gccattgaga tgttagatag gcgccatact cacttttgcc
      361 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
      421 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
      481 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
      541 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
      601 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
      661 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
      721 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcttaaa
      781 agcagcataa cctttttccg tgatggtaac ttcaagatcg attcaagggg ttgatgtagc
      841 agtgttaaga gagcatgatg aagccgggaa ggtttagaga agtctcgttc cgacagttgg
      901 caacataata tgctaaaggc ctcgatcccg caagaggccc ggcagtaccg gcataaccaa
      961 gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga
     1021 tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag
     1081 cttatcgatt atgtcttttg cgcagtcggc ttaaaccagt tt
//
