LOCUS       sequence-125017-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x Target 1 0gap minus SV40 Ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-294
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    206..227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(242..247,248..265,266..272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    280..296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     285..307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     304..320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     304..320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     663..682
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgga tctctcgagt taatttcgct taatttcgcg
       61 gatccggacc acttcccctc cagcacctcc tgcagcttct tcaagatccg gaccacttcc
      121 cctccagcac ctcctgcagc ttcttcaaga tccgctggca cgacaggttt cccgactgga
      181 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      241 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      301 acacaggaaa cagctatgac catgattacg ccaagcttgg gctgcaggtc gagggatctc
      361 cataagagaa gagggacagc tatgactggg agtagtcagg agaggaggaa aaatctggct
      421 agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa aaaataacac aaaacaaaat
      481 ataaaaaaaa tctaacctca agtcaaggct tttctatgga ataaggaatg gacagcaggg
      541 ggctgtttca tatactgatg acctctttat agccaacctt tgttcatggc agccagcata
      601 tgggcatatg ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc
      661 ctcccatatg tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa
      721 ataaatttcc
//
