LOCUS       sequence-125021-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Suicide plasmid for efficient gene mutation in Gram-positive 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdelhamed H, Lawrence ML, Karsi A
  TITLE     A novel suicide plasmid for efficient gene mutation in Listeria 
            monocytogenes.
  JOURNAL   Plasmid. 2015 May 31;81:1-8. doi: 10.1016/j.plasmid.2015.05.003.
  PUBMED    26038185
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2015-05-31"; volume: "81:1-8. doi"; pages: " 
            10.1016/j.plasmid.2015.05.003"
COMMENT     Sequence Label: pHoss1
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     302..321
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    419..437
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    504..522
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
       61 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
      121 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
      181 cttctaaagg gcaaaagtga gtatggcgcc tatctaacat ctcaatggct aaggcgtcga
      241 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
      301 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
      361 tgttaatcac tttactttta tctaatctag acatcattaa ttcctccttt ttgttgacac
      421 tctatcattg atagagttat ttgtcaaact agttttttat ttggatcccc tcgagttcat
      481 gaaaaactaa aaaaaatatt gacactctat cattgataga gtataattaa aataagcttg
      541 atgggaatat gataatagaa ataccattac caacaccgaa ctgagtgatt tgatcaccaa
      601 gccatattaa gaaagcagtt cctgctgtca aaactagtgc tattaataaa taactcataa
      661 ttgactgatt gataatcagc gcacctttga gataattatt aaattggaat gccataccta
      721 tagattggat aaatgctaaa gaaattgcta aataacgagt aacgttattt aactttcttc
      781 tacctacttc accttgtttt gcccattctg agaatttagg gacaatatcc atttgtaata
      841 attgcattac gattgatgca gtgatgtagg gtacaatacc cattgcaaaa atagaaaatc
      901 gtttcaaggc tccgccacca aaagtattta ataactcagt ggcaccttga gaaccttggg
      961 gattatcaaa agctgcagga tttactcctg gagctggtat ataagtccct attttaaaaa
     1021 ttactaacat tgctagtgtg aagaaaatct tgttacgaac ctcttttgtt ctaaagaagt
     1081 tcacaagggt ttgaatcatt agatcccatg gtacccggga gctcgaattc tagaagcttc
     1141 tgcaga
//
