LOCUS       sequence-125035-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express recepter TVA for envA with AAV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Menegas W, Bergan JF, Ogawa SK, Isogai Y, Umadevi Venkataraju K, 
            Osten P, Uchida N, Watabe-Uchida M
  TITLE     Dopamine neurons projecting to the posterior striatum form an 
            anatomically distinct subclass.
  JOURNAL   Elife. 2015 Aug 31;4. doi: 10.7554/eLife.10032.
  PUBMED    26322384
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-31"; volume: "4. doi"; pages: " 10.7554/eLife.10032"
COMMENT     Sequence Label: pAAV-EF1a-flex-TVA
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..83)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(134..167)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             624..647
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    667..700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    751..784
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          824..1027
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(847..867)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 caattttgta atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta
       61 tagcatacat tatacgaagt tattctttgc accattctaa agaataacag tgataatttc
      121 tgggttaagg caaataactt cgtataggat actttatacg aagttatgct agagcttgat
      181 gtggtggtac cgacatggcg cggctgctgc ccgcgctgct gctgctgctg ctgcccggta
      241 acgtgaccgg taacgggtcc ggtaacggtt ctttgtcccg ttgccccccc ggtcagttcc
      301 gctgctcgga gccgcccggt gcccacgggg agtgttaccc gcaggactgg ctgtgcgacg
      361 gacaccccga ctgcgacgac gggcgggacg agtggggctg cgggaccagc gcgatccccg
      421 cggtgcccac ggacaacggc acagaggctc ccactgcccc tgctcctgga cgtgctctgc
      481 cagccaggaa tcacggccgc atgtggatgc tgatcactgc agtgctcctg tgctgcctgg
      541 tagctgtggg tggtatcgct gcatggggga agtccaaagc aaaaagcagg tctgacatct
      601 tcagtcttga aagcgcatcc aaggactaca aggacgacga cgacaagtag gatccactag
      661 cgcgccataa cttcgtataa tgtatgctat acgaagttat taagaggttt catattgcta
      721 atagcagcta caatccagct accattctgc ataacttcgt ataaagtatc ctatacgaag
      781 ttattccgga gtcgactcta gaggatccgg tacctcacga cacctgaaat ggaagaaaaa
      841 aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa
      901 attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag
      961 tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa
     1021 agctcgagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaa
//
