LOCUS       sequence-125047-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as 
            gene of interest for integration on cxtTi10816, Mos Chr IV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR04
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..110)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    139..172
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      186..509
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
ORIGIN
        1 ttgtgtccgt tgacgtctcc gtcgagctcg acgaggattg ggacgactcc ggtgaagagc
       61 tcctctccct tggacatttt ttctaccggt accttacgct tcttctttgg agcagtcatt
      121 ttttctggat cctctagaat aacttcgtat aatgtatgct atacgaagtt atgcggccgc
      181 gataaatgca aaatcctttc aagcattccc ttcttctcta tcactcttct ttctttttgt
      241 caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat gactttttat
      301 agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga ttctgtctga
      361 tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg aaaggttgag
      421 aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac aaaaagagga
      481 aactttattg tgccgccaag aaaaaagtct cattagttga gcttgtgtcc gagcttggat
      541 gggaggtcgc agtaacgggc gacggcgacc tcgtgttgct cgacgtaggt ctcgttgttg
      601 gcctccttga tacgctctag acggtagtca acgtagtaga ctcctggcat cttgaggttc
      661 ttggctggct tcttggaacg gtaggtggtc tgaaaattta aatatgtatg gttagttagt
      721 accgaactgt
//
