LOCUS       sequence-125155-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Mito-7
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..76
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             110..802
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc ggcggctccc
       61 agtgccgcgc gccaagatcc attcgttggg ggatccaccg gtcgccacca tgagcgagct
      121 gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
      181 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgaagatcaa
      241 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
      301 cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
      361 ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg tgctgaccgc
      421 tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga tcaacggggt
      481 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg aggccaacac
      541 cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg ccctgaagct
      601 cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca agaaacccgc
      661 taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa gaatcaagga
      721 ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaagt actgcgacct
      781 ccctagcaaa ctggggcaca gagggcatgg caccggcagc accggcagcg gcagctccgg
      841 taccgcctcc tccgaggaca acaacatggc catgagcgag ctgatcaagg agaacatgca
//
