LOCUS       sequence-125292-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 with His, MBP, and Strep tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  TITLE     Gerard Cagney unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NM-01
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..84)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cttctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtca
       61 tttttcgaac tgcgggtggc tccagtcacc tcctagctga ctcaaatcaa tgcgtgtttc
      121 ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt ttgtagacgt
      181 atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag ctccaagatt
      241 cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt tgtctctatg
      301 tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa cacgcttaga
      361 aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct ccacaaacaa
      421 ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac tagctaaata
      481 taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta attctccggc
      541 actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt taggtagttt
      601 aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt caatcggatt
      661 tttttcaaag gaacttcttt tccataattt gtgatcccta gtaactcttt taacggattt
      721 taacttcttc gatttccctt tttccacctt agcaaccact aggactgaat aagctaccgt
      781 tggactatca aaaccaccat atttttttgg atcccagtct tttttacgag caataagctt
      841 gtccgaattt ctttttggta aaattgactc cttggagaat ccgcctgtct gtacttctgt
      901 tttctggaca atattgactt ggggcatgga caatactttg gcgcactggg gcaaaatctc
      961 gcccctttaa tccagacaat tcctcagttt ccccataagt tcgattaaaa gggcgtttgg
     1021 cgaatctctc catttgca
//
