LOCUS       sequence-125300-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD pduP1-18-gfpmut2-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: EYK054
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..28
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             93..803
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             93..803
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(120..148)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     743..763
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcgaattcga gctcttaaag aggagaaagg tcatgaatac ttctgaactc gaaaccctga
       61 ttcgcaccat tcttagcgag caattatcta gaagtaaagg agaagaactt ttcactggag
      121 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      181 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      241 gaaaactacc tgttccatgg ccaacacttg tcactacttt cgcgtatggt cttcaatgct
      301 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
      361 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      421 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      481 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      541 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
      601 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      661 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
      721 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
      781 atggcatgga tgaactatac aaagtcgacg cagcaaacga cgaaaactac gctttagcag
      841 cttaagcatg caagcttggc tgt
//
