LOCUS       sequence-125308-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD pduD1-20-gfpmut2-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ038
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..749)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(39..749)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(79..99)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     694..722
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgctaaag cgtagttttc gtcgtttgct gcgtcgactt tgtatagttc atccatgcca
       61 tgtgtaatcc cagcagctgt tacaaactca agaaggacca tgtgatctct cttttcgttg
      121 ggatctttcg aaagggcaga ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg
      181 ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgct tccatcttca
      241 atgttgtgtc taattttgaa gttaactttg attccattct tttgtttgtc tgccatgatg
      301 tatacattgt gtgagttata gttgtattcc aatttgtgtc caagaatgtt tccatcttct
      361 ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc aaacttgact
      421 tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc ctgtacataa
      481 ccttcgggca tggcactctt gaaaaagtca tgctgtttca tatgatctgg gtatctcgca
      541 aagcattgaa gaccatacgc gaaagtagtg acaagtgttg gccatggaac aggtagtttt
      601 ccagtagtgc aaataaattt aagggtaagt tttccgtatg ttgcatcacc ttcaccctct
      661 ccactgacag aaaatttgtg cccattaaca tcaccatcta attcaacaag aattgggaca
      721 actccagtga aaagttcttc tcctttactt ctagacttca tatcgcggag tacgtcttca
      781 attatctggc gcagcaattt t
//
