LOCUS       sequence-125360-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a 
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPAt2S3 (GB0029)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      448..457
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      452..461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(491..510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(492..510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(517..534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(517..533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(526..548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(761..780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catatggtcg acctgcaggc ggccgcgaat tcactagtga ttgggcgatg agtggtctcg
       61 ggaggaaacc aaattaacat agggttttta tttaaataaa agtttaacct tctttttaaa
      121 aaattgttca tagtgtcatg tcagaacaag agctacaaat cacacatagc atgcataagc
      181 ggagctatga tgagtggtat tgttttgttc gtcacttgtc actcttttcc aacacataat
      241 cccgacaaca acgtaagagc atctctctct ctccacacac actcatgcat gcatgcattc
      301 ttacacgtga ttgccatgca aatctccttt ctcacctata aatacaaacc aacccttcac
      361 tacactcttc actcaaacca aaacaagaaa acatacacaa atagcaaaac aatgcgagac
      421 cactcatcgc ccaatcgaat tcccgcggcc gccatggcgg ccgggagcat gcgacgtcgg
      481 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
      541 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      601 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      661 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      781 tcctttctcg ccacgttcgc cggctttcc
//
