LOCUS       sequence-125398-        9733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP35
FEATURES             Location/Qualifiers
     source          1..9733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             465..487
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             507..524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             528..560
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(564..572,573..587)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    1302..1426
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1451..1456,1457..1474,1475..1481)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1601..1620)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2594..2899
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2778..2797
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2940..3064)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3756..3785
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3823..3839)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3824..3840)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3864..3883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3865..3883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      4031..4218
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4086..4110)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4230..4247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4230..4246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4239..4261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4682..6028
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6139..6942)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6943..7158)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7183..7201)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7269..7373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7374..7442,7443..8234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7592..7611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8405..8993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8894..8913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9147..9164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9281..9302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9317..9322,9323..9340,9341..9347)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9355..9371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9360..9382
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9379..9395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9379..9395
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
       61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
      121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
      181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
      241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
      301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
      361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
      421 aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt taactttaag
      481 aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
      541 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgggcctcg
      601 aggtttctaa aggtgaagaa ttgtttacgg gcgtcgtccc gatcctcgtg gaactcgacg
      661 gggatgttaa cgggcataag ttttcggtca gcggggaagg ggagggggac gcgacgtatg
      721 ggaagctcac tctcaagctg atctgtacga cggggaaact cccggtcccg tggccgacgc
      781 tggtcacgac gctgggatac gggctccaat gctttgcgag gtatccggac cacatgaaac
      841 agcatgactt tttcaaatcg gcgatgccgg agggatacgt gcaggaacgg acgatctttt
      901 tcaaagacga tgggaactat aagacgcggg cggaagtcaa gtttgaaggg gacacgctcg
      961 tcaaccggat cgaactcaag gggattgact tcaaagagga tgggaacata ctcggccata
     1021 agctcgaata caattacaac tcgcataacg tatacatcac cgcggataag caaaagaacg
     1081 ggatcaaagc caatttcaaa atccggcata acatagagga tgggggggtc caactggcgg
     1141 atcactatca gcaaaacacg ccgatagggg atgggccggt cctcctcccg gataaccatt
     1201 acctctcgta ccaaagcgcg ctctcgaagg acccgaatga gaaacgggac cacatggttc
     1261 tcctggagtt cgtcacggcg gcgggcatag gtaccgatat cacaagtttg tacaaaaaag
     1321 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     1381 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     1441 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1501 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1561 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1621 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1681 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1741 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1801 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1861 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1921 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1981 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2041 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2101 tcaggttcat catgccgttt gtgatgggct tccatgtcgg cagaatgctt aatgaattac
     2161 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     2221 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2281 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2341 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2401 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2461 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2521 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaacttttgc tgacgagaac
     2581 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     2641 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     2701 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     2761 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     2821 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     2881 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     2941 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     3001 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     3061 tggtgatatc gactagtgtg agcaagggcg aggagaccac aatgggcgta atcaagcccg
     3121 acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc gtgatcgagg
     3181 gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag gtgaaggagg
     3241 gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac ggcaacaggg
     3301 ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc cccgagggct
     3361 actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg aagtccgaca
     3421 tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag aacttccccc
     3481 ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc gagaggatgt
     3541 acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg gagggcggcg
     3601 gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg aagctgcccg
     3661 actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac tacaacaagg
     3721 tgaccgttta cgagagcgcc gtggcccgca actccgaaca aaaattgata agtgaggaag
     3781 atttataagg gccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
     3841 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgg atccagcttt
     3901 ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt
     3961 gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac
     4021 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     4081 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaraat tcttattctt
     4141 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     4201 tgaccacacc tctaccggca tgccaattca ctggccgtcg ttttacaacg tcgtgactgg
     4261 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4321 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4381 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4441 atcggatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     4501 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     4561 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     4621 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     4681 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     4741 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     4801 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     4861 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     4921 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     4981 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     5041 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     5101 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     5161 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     5221 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     5281 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     5341 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     5401 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     5461 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     5521 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     5581 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     5641 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
     5701 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     5761 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     5821 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     5881 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     5941 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     6001 atgctatcat ttcctttgat attggatcga tccgatgata agctgtcaaa catgagaatt
     6061 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     6121 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     6181 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     6241 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     6301 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     6361 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     6421 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
     6481 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
     6541 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     6601 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     6661 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     6721 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     6781 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     6841 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     6901 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     6961 gcatgttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     7021 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     7081 ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa
     7141 aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
     7201 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9241 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9301 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9361 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttcctgaaa
     9421 cggagaaaca taaacaggca ttgctgggat cacccataca tcactctgtt ttgcctgacc
     9481 ttttccggta atttgaaaac aaacccggtc tcgaagcgga gatccggcga taattaccgc
     9541 agaaataaac ccatacacga gacgtagaac cagccgcaca tggccggaga aactcctgcg
     9601 agaatttcgt aaactcgcgc gcattgcatc tgtatttcct aatgcggcac ttccaggcct
     9661 cgatcgagac cgtttatcca ttgctttttt gttgtctttt tccctcgttc acagaaagtc
     9721 tgaagaagct ata
//
