LOCUS       sequence-125405-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA expression construction in a 3rd generation lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-sgRNA-Lib
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(499..623)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(652..671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(695..943)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(753..772)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(923..943)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgat acagtagaaa
      121 ttacagaaac tttatcacgt ttagtaagta tagaggctga aaatccagat gaagccgaac
      181 gacttgtaag agaaaagtat aagagttgtg aaattgttct tgatgcagat gattttcagg
      241 actatgacac tagcgtatat gaataggtag atgtttttat tttgtcacac aaaaaagagg
      301 ctcgcacctc tttttcttat ttctttttat gatttaatac ggcattgagg acaatagcga
      361 gtaggctgga tacgacgatt ccgtttgaga agaacatttg gaaggctgtc ggtcgactaa
      421 gttggcagca tcacccgaag aacatttgga aggctgtcgg tcgactacag gtcactaata
      481 ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
      541 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      601 tcagcttttt tgtacaaact tgttgatagc ttaactagcc agcttgggtc tccctatagt
      661 gagtcgtatt aatttcgata agccagcgtc tctcggtgtt tcgtcctttc cacaagatat
      721 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
      781 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
      841 gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt
      901 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcttcctgc cagaccttgg
      961 tacctctaga gcgggcccgg tactaaccaa actggatctc tgctgtccct gtaataaacc
     1021 cgaaaatttt gaa
//
