LOCUS       sequence-125408-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA expression construction in a 3rd generation lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-sgRNA-Lib
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     227..246
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        227..245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    275..399
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(930..949)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      181 tcttgtggaa aggacgaaac accgagagac gctggcttat cgaaattaat acgactcact
      241 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagctgaacg
      301 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
      361 tacataatac tgtaaaacac aacatatcca gtcactatga atcaactact tagatggtat
      421 tagtgacctg tagtcgaccg acagccttcc aaatgttctt cgggtgatgc tgccaactta
      481 gtcgaccgac agccttccaa atgttcttct caaacggaat cgtcgtatcc agcctactcg
      541 ctattgtcct caatgccgta ttaaatcata aaaagaaata agaaaaagag gtgcgagcct
      601 cttttttgtg tgacaaaata aaaacatcta cctattcata tacgctagtg tcatagtcct
      661 gaaaatcatc tgcatcaaga acaatttcac aactcttata cttttctctt acaagtcgtt
      721 cggcttcatc tggattttca gcctctatac ttactaaacg tgataaagtt tctgtaattt
      781 ctactgtatc gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag
      841 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
      901 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      961 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
     1021 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctg
//
