LOCUS       sequence-125486-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Beta1-GFP integrin for retroviral transfection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Parsons M, Messent AJ, Humphries JD, Deakin NO, Humphries MJ
  TITLE     Quantification of integrin receptor agonism by fluorescence lifetime
            imaging.
  JOURNAL   J Cell Sci. 2008 Feb 1;121(Pt 3):265-71. doi: 10.1242/jcs.018440.
  PUBMED    18216331
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2008-02-1"; volume: "121(Pt 3):265-71. doi"; pages: " 
            10.1242/jcs.018440"
COMMENT     Sequence Label: Beta1-GFP pFB-Neo
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..64)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(169..198)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(363..382)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     592..609
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(762..783)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcccatggaa ccgcgtggca
       61 ccagggacgg gtgcagcgag ggggcagcca tggagcctcg ccccgtcggg gaggtgaggg
      121 aggagttcac ctgggtggag aaatcgagcg gcaggaaagt ttggtgtcca agtcttcttc
      181 agaaataagt ttttgttcca tattatcgat cgtgtttttc aaaggaaaac cacgtccccg
      241 tggttcgggg ggcctagacg ttttttaacc tcgactaaac acatgtaaag catgtgcacc
      301 gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt
      361 tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc
      421 actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc
      481 acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc
      541 ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt
      601 tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg
      661 ccctcacatt gccaaaagac ggcaatatgg tggaaaataa ccggatcgcg gccgctttac
      721 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      781 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      841 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      901 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      961 ttctgcttgt cggccatgat atagacgttg tggctgttgt aattgtactc cagcttgtgg
     1021 cccagaatgt tgccgtcctc cttgaaatca atgcccttca gctcgatgcg gttcaccagg
     1081 gt
//
