LOCUS       sequence-125539-        3697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a 
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPAtUbq10  (GB0223)
FEATURES             Location/Qualifiers
     source          1..3697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(747..765)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(748..765)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(783..799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(783..799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(796..818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    807..823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(831..837,838..855,856..861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    876..897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1014..1031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1185..1773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1265..1284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1944..2735,2736..2804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2567..2586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2805..2909)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2987..3442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3124..3145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3336..3355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3568..3590
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3582..3599
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3583..3599
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3606..3625
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3606..3624
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3655..3664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3659..3668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggaggt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
       61 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
      121 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
      181 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
      241 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
      301 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
      361 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
      421 ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc
      481 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
      541 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
      601 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagaatgcg agaccactca
      661 tcgcccaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca
      721 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
      781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1141 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1201 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1261 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1321 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1381 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1441 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1501 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1561 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1621 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1681 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1741 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1801 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1861 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1921 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1981 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2041 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2101 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2161 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2221 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2281 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2341 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2401 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2461 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2521 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2581 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2641 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2701 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2761 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2821 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2881 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg
     2941 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     3001 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3061 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3121 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3181 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3241 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3301 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3361 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3421 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     3481 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3541 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3601 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
     3661 atggcggccg cgggaattcg attgggcgat gagtggt
//
