LOCUS       sequence-125568-        2736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Source of psbA promoter of Amaranthus hybridus, used as a strong 
            promoter of cyanobacterial genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Elhai J, Wolk CP
  TITLE     A versatile class of positive-selection vectors based on the 
            nonviability of palindrome-containing plasmids that allows cloning 
            into long polylinkers.
  JOURNAL   Gene. 1988 Aug 15;68(1):119-38.
  PUBMED    2851487
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1988-08-15"; volume: "68(1)"; pages: "119-38"
COMMENT     Sequence Label: pRL439
FEATURES             Location/Qualifiers
     source          1..2736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(105..121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(105..121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(118..140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(153..159,160..177,178..183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    198..219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(336..353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(507..1095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(587..606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1266..2057,2058..2126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1889..1908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2127..2231)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2299..2317
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2355..2377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2477..2496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2690..2712
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2704..2721
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2705..2721
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggtcgactc tagaggatct caatgaatat tggttgacac gggcgtataa gacatgttat
       61 actgttgaat aacaaggacg gatccccggg aattcgtaat catggtcata gctgtttcct
      121 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      181 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      241 gctttccagt cgggaaacct gtcgtgccag ctggattaat gaatcggcca acgcgcgggg
      301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
      721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
      961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2281 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     2341 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2401 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2461 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     2521 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     2581 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     2641 aaggggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     2701 cgttgtaaaa cgacggccag tgccaagctt ggctgc
//
