LOCUS       sequence-125602-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Agrobacterium tumefaciens Nopaline synthase terminator 
            as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTnos (GB0037)
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      85..337
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(372..390)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     regulatory      595..604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(638..657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(639..657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(664..681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(664..680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(673..695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccatatggtc gacctgcagg cggccgcgaa ttcactagtg attgggcgat gagtggtctc
       61 ggcttggaat ggatcttcga tcccgatcgt tcaaacattt ggcaataaag tttcttaaga
      121 ttgaatcctg ttgccggtct tgcgacgatt atcatataat ttctgttgaa ttacgttaag
      181 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
      241 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
      301 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattgccaag ctaattcttg
      361 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      421 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      481 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      541 ataatgggac cgactcgcgc tcgagaccac tcatcgccca atcgaattcc cgcggccgcc
      601 atggcggccg ggagcatgcg acgtcgggcc caattcgccc tatagtgagt cgtattacaa
      661 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      721 tcgccttgca gcacatccc
//
