LOCUS       sequence-125616-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized Lb2Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY011 (pcDNA3.1-hLb2Cpf1)
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgccacc atgtactatg agtccctgac
      121 caagcagtac cccgtgtcta agacaatccg gaatgagctg atccctatcg gcaagacact
      181 ggataacatc cgccagaaca atatcctgga gagcgacgtg aagcggaagc agaactacga
      241 gcacgtgaag ggcatcctgg atgagtatca caagcagctg atcaacgagg ccctggacaa
      301 ttgcaccctg ccatccctga agatcgccgc cgagatctac ctgaagaatc agaaggaggt
      361 gtctgacaga gaggatttca acaagacaca ggacctgctg aggaaggagg tggtggagaa
      421 gctgaaggcc cacgagaact ttaccaagat cggcaagaag gacatcctgg atctgctgga
      481 gaagctgcct tccatctctg aggacgatta caatgccctg gagagcttcc gcaactttta
      541 cacctatttc acatcctaca acaaggtgcg ggagaatctg tattctgata aggagaagag
      601 ctccacagtg gcctacagac tgatcaacga gaatttccca aagtttctgg acaatgtgaa
      661 gagctatagg tttgtgaaaa ccgcaggcat cctggcagat ggcctgggag aggaggagca
      721 ggactccctg ttcatcgtgg agacattcaa caagaccctg acacaggacg gcatcgatac
      781 ctacaattct caagtgggca agatcaactc tagcatcaat ctgtataacc agaagaatca
      841 gaaggccaat ggcttcagaa a
//
