LOCUS       sequence-125623-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized EeCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY013 (pcDNA3.1-hEeCpf1)
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttctcgta ccgcttgttc tggatgaagt ccagccactc tgcgtgtggc agcttcaggc
      241 aattcctatc aaagccgtcc tcggtccact cgctcttgat cttcagcacc tcatacaggc
      301 ccttcagggc gatacagtag gcgccgttgg catcggcgtc ctttggcatc ttgcactcct
      361 tatcgtcaga ctccttatag ttatcggagt caaagaactc gccctcatca ttgatcacag
      421 ggctgatgat cttgtcgtaa gagatgccgt tctcctgctc ctcggcctct gtataggaat
      481 tgcgcatctg cacggtcagc ttatacaggc tcagcagctg ggcaaagaac tcgctcttct
      541 tatcctcgtc catcttctcc atatcgatcc tgatatcgtg gccgtcggcg tagttgatct
      601 cattgtcctc cagcagcagc ttgattgtct ctgtcagatt gatgctcttt gtcttgccgg
      661 tgcgcctggc attgttgaac tcagactgca gtctctcgcc gtttgtatac acggtccact
      721 gtgtcttgcc cattgtgatg ttgtaggtat cgaagttgtt gtagtcgaag ccaaagctga
      781 acatatcctt ctcggcacag tatctgatct cgtcaaa
//
