LOCUS       sequence-125646-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal plasmid encoding  5 gRNAS targeting human OCT4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: GG-EBNA-OCT4
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        58..306
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        327..402
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     434..450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        451..699
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 gactctagag gatctcgagc tcaagcttcg aattccccct gtaaaacgac ggccagtgag
       61 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      121 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      181 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      241 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      301 aacaccgggg ggagaaactg aggcgagttt tagagctaga aatagcaagt taaaataagg
      361 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc atggtcatag
      421 ctgtttcctc atggtaaaac gacggccagt gagggcctat ttcccatgat tccttcatat
      481 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
      541 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
      601 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
      661 ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggtggtggca atggtgtctg
      721 gttttagagc tagaaatagc aagttaaaat aaggctagtc cg
//
