LOCUS       sequence-125709-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of the Blue fluorescente protein as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pBFP (GB0025)
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..767)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 ccatatggtc gacctgcagg cggccgcgaa ttcactagtg attgggcgat gagtggtctc
       61 gaagctcaat taagcttgtg ccccagtttg ctagggaggt cgcagtatct ggccactgcc
      121 acctcgtgct gctcgacgta ggtttcgttg ttggcctcct tgattctttc cagtctgtag
      181 tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga tctatatgtg
      241 gtcttgatgt ttgcgatcag atggctcccg cccacgagct tcagggccat gtcgtttctg
      301 ccttccaggc cgccgtcagc ggggtacagc gtttcggtga aggcctccca gccgagtgtt
      361 ttcttctgca tcacagggcc gttggatgtg aagttcaccc ctctgatctt gacgttgtag
      421 atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg
      481 tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttgaa gaagtcgggg
      541 atgccctggg tgtggttgat gaaggtcttg ctgccgtaga ggaagctagt agccaggatg
      601 tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc
      661 tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt gatggttgtc cacggtgccc
      721 tccatgtaca gcttcatgtg catgttctcc ttaatcagct cgctcattcg agaccactca
      781 tcgcc
//
