LOCUS       sequence-125825-        5672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human FLT3 [NM_004119.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E092 Hs.FLT3-nostop
FEATURES             Location/Qualifiers
     source          1..5672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3163..3262)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3639..4430
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4523..5111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5012..5031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5265..5282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5441..5468)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5560..5646)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc ggcgttggcg cgcgacggcg gccagctgcc gctgctcgtt gttttttctg
      241 caatgatatt tgggactatt acaaatcaag atctgcctgt gatcaagtgt gttttaatca
      301 atcataagaa caatgattca tcagtgggga agtcatcatc atatcccatg gtatcagaat
      361 ccccggaaga cctcgggtgt gcgttgagac cccagagctc agggacagtg tacgaagctg
      421 ccgctgtgga agtggatgta tctgcttcca tcacactgca agtgctggtc gatgccccag
      481 ggaacatttc ctgtctctgg gtctttaagc acagctccct gaattgccag ccacattttg
      541 atttacaaaa cagaggagtt gtttccatgg tcattttgaa aatgacagaa acccaagctg
      601 gagaatacct actttttatt cagagtgaag ctaccaatta cacaatattg tttacagtga
      661 gtataagaaa taccctgctt tacacattaa gaagacctta ctttagaaaa atggaaaacc
      721 aggacgccct ggtctgcata tctgagagcg ttccagagcc gatcgtggaa tgggtgcttt
      781 gcgattcaca gggggaaagc tgtaaagaag aaagtccagc tgttgttaaa aaggaggaaa
      841 aagtgcttca tgaattattt gggatggaca taaggtgctg tgccagaaat gaactgggca
      901 gggaatgcac caggctgttc acaatagatc taaatcaaac tcctcagacc acattgccac
      961 aattatttct taaagtaggg gaacccttat ggataaggtg caaagctgtt catgtgaacc
     1021 atggattcgg gctcacctgg gaattagaaa acaaagcact cgaggagggc aactactttg
     1081 agatgagtac ctattcaaca aacagaacta tgatacggat tctgtttgct tttgtatcat
     1141 cagtggcaag aaacgacacc ggatactaca cttgttcctc ttcaaagcat cccagtcaat
     1201 cagctttggt taccatcgta gaaaagggat ttataaatgc taccaattca agtgaagatt
     1261 atgaaattga ccaatatgaa gagttttgtt tttctgtcag gtttaaagcc tacccacaaa
     1321 tcagatgtac gtggaccttc tctcgaaaat catttccttg tgagcaaaag ggtcttgata
     1381 acggatacag catatccaag ttttgcaatc ataagcacca gccaggagaa tatatattcc
     1441 atgcagaaaa tgatgatgcc caatttacca aaatgttcac gctgaatata agaaggaaac
     1501 ctcaagtgct cgcagaagca tcggcaagtc aggcgtcctg tttctcggat ggatacccat
     1561 taccatcttg gacctggaag aagtgttcag acaagtctcc caactgcaca gaagagatca
     1621 cagaaggagt ctggaataga aaggctaaca gaaaagtgtt tggacagtgg gtgtcgagca
     1681 gtactctaaa catgagtgaa gccataaaag ggttcctggt caagtgctgt gcatacaatt
     1741 cccttggcac atcttgtgag acgatccttt taaactctcc aggccccttc cctttcatcc
     1801 aagacaacat ctcattctat gcaacaattg gtgtttgtct cctcttcatt gtcgttttaa
     1861 ccctgctaat ttgtcacaag tacaaaaagc aatttaggta tgaaagccag ctacagatgg
     1921 tacaggtgac cggctcctca gataatgagt acttctacgt tgatttcaga gaatatgaat
     1981 atgatctcaa atgggagttt ccaagagaaa atttagagtt tgggaaggta ctaggatcag
     2041 gtgcttttgg aaaagtgatg aacgcaacag cttatggaat tagcaaaaca ggagtctcaa
     2101 tccaggttgc cgtcaaaatg ctgaaagaaa aagcagacag ctctgaaaga gaggcactca
     2161 tgtcagaact caagatgatg acccagctgg gaagccacga gaatattgtg aacctgctgg
     2221 gggcgtgcac actgtcagga ccaatttact tgatttttga atactgttgc tatggtgatc
     2281 ttctcaacta tctaagaagt aaaagagaaa aatttcacag gacttggaca gagattttca
     2341 aggaacacaa tttcagtttt taccccactt tccaatcaca tccaaattcc agcatgcctg
     2401 gttcaagaga agttcagata cacccggact cggatcaaat ctcagggctt catgggaatt
     2461 catttcactc tgaagatgaa attgaatatg aaaaccaaaa aaggctggaa gaagaggagg
     2521 acttgaatgt gcttacattt gaagatcttc tttgctttgc atatcaagtt gccaaaggaa
     2581 tggaatttct ggaatttaag tcgtgtgttc acagagacct ggccgccagg aacgtgcttg
     2641 tcacccacgg gaaagtggtg aagatatgtg actttggatt ggctcgagat atcatgagtg
     2701 attccaacta tgttgtcagg ggcaatgccc gtctgcctgt aaaatggatg gcccccgaaa
     2761 gcctgtttga aggcatctac accattaaga gtgatgtctg gtcatatgga atattactgt
     2821 gggaaatctt ctcacttggt gtgaatcctt accctggcat tccggttgat gctaacttct
     2881 acaaactgat tcaaaatgga tttaaaatgg atcagccatt ttatgctaca gaagaaatat
     2941 acattataat gcaatcctgc tgggcttttg actcaaggaa acggccatcc ttccctaatt
     3001 tgacttcgtt tttaggatgt cagctggcag atgcagaaga agcgatgtat cagaatgtgg
     3061 atggccgtgt ttcggaatgt cctcacacct accaaaacag gcgacctttc agcagagaga
     3121 tggatttggg gctactctct ccgcaggctc aggtcgaaga ttcgtcaact ttcttgtaca
     3181 aagttggcat tataggaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3241 gtcaaaataa aatcattatt tgcatgcaag cttagatcct ctagaccagc caggacagaa
     3301 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     3361 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     3421 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     3481 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     3541 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     3601 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     3661 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     3721 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     3781 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     3841 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     3901 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     3961 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     4021 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     4081 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     4141 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     4201 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     4261 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     4321 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     4381 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     4441 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     4501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     4921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5161 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5221 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5281 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5341 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5401 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     5461 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     5521 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     5581 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     5641 tatttgatgc ctggcagttc cctactctcg cg
//
