LOCUS       sequence-125858-        3776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3776)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E125 Hs.MAPK1
FEATURES             Location/Qualifiers
     source          1..3776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      184..193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1230..1265
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     protein_bind    complement(1267..1366)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1743..2534
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2627..3215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3116..3135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3369..3386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3545..3572)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3664..3750)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc ggcggcggcg gcggcgggcg cgggcccgga gatggtccgc gggcaggtgt
      241 tcgacgtggg gccgcgctac accaacctct cgtacatcgg cgagggcgcc tacggcatgg
      301 tgtgctctgc ttatgataat gtcaacaaag ttcgagtagc tatcaagaaa atcagcccct
      361 ttgagcacca gacctactgc cagagaaccc tgagggagat aaaaatctta ctgcgcttca
      421 gacatgagaa catcattgga atcaatgaca ttattcgagc accaaccatc gagcaaatga
      481 aagatgtata tatagtacag gacctcatgg aaacagatct ttacaagctc ttgaagacac
      541 aacacctcag caatgaccat atctgctatt ttctctacca gatcctcaga gggttaaaat
      601 atatccattc agctaacgtt ctgcaccgtg acctcaagcc ttccaacctg ctgctcaaca
      661 ccacctgtga tctcaagatc tgtgactttg gcctggcccg tgttgcagat ccagaccatg
      721 atcacacagg gttcctgaca gaatatgtgg ccacacgttg gtacagggct ccagaaatta
      781 tgttgaattc caagggctac accaagtcca ttgatatttg gtctgtaggc tgcattctgg
      841 cagaaatgct ttctaacagg cccatctttc cagggaagca ttatcttgac cagctgaacc
      901 acattttggg tattcttgga tccccatcac aagaagacct gaattgtata ataaatttaa
      961 aagctaggaa ctatttgctt tctcttccac acaaaaataa ggtgccatgg aacaggctgt
     1021 tcccaaatgc tgactccaaa gctctggact tattggacaa aatgttgaca ttcaacccac
     1081 acaagaggat tgaagtagaa caggctctgg cccacccata tctggagcag tattacgacc
     1141 cgagtgacga gcccatcgcc gaagcaccat tcaagttcga catggaattg gatgacttgc
     1201 ctaaggaaaa gctcaaagaa ctaatttttg aagagactgc tagattccag ccaggataca
     1261 gatcttaatc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     1321 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     1381 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1441 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1501 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1561 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1621 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1681 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1741 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1801 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1861 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1921 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1981 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2041 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2101 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2161 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2221 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2281 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2341 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2401 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2461 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2521 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2581 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2641 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2701 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2761 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2821 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2881 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2941 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3001 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3061 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3121 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3181 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3241 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3301 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3361 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3421 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3481 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3541 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3601 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3661 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3721 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
