LOCUS       sequence-125897-        5897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CA [NM_006218.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5897)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E164 Hs.PIK3CA-nostop
FEATURES             Location/Qualifiers
     source          1..5897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3388..3487)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3864..4655
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4748..5336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5237..5256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5490..5507
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5666..5693)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5785..5871)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc tccacgacca tcatcaggtg aactgtgggg catccacttg atgcccccaa
      241 gaatcctagt agaatgttta ctaccaaatg gaatgatagt gactttagaa tgcctccgtg
      301 aggctacatt aataaccata aagcatgaac tatttaaaga agcaagaaaa taccccctcc
      361 atcaacttct tcaagatgaa tcttcttaca ttttcgtaag tgttactcaa gaagcagaaa
      421 gggaagaatt ttttgatgaa acaagacgac tttgtgacct tcggcttttt caaccctttt
      481 taaaagtaat tgaaccagta ggcaaccgtg aagaaaagat cctcaatcga gaaattggtt
      541 ttgctatcgg catgccagtg tgtgaatttg atatggttaa agatccagaa gtacaggact
      601 tccgaagaaa tattctgaac gtttgtaaag aagctgtgga tcttagggac ctcaattcac
      661 ctcatagtag agcaatgtat gtctatcctc caaatgtaga atcttcacca gaattgccaa
      721 agcacatata taataaatta gataaagggc aaataatagt ggtgatctgg gtaatagttt
      781 ctccaaataa tgacaagcag aagtatactc tgaaaatcaa ccatgactgt gtaccagaac
      841 aagtaattgc tgaagcaatc aggaaaaaaa ctcgaagtat gttgctatcc tctgaacaac
      901 taaaactctg tgttttagaa tatcagggca agtatatttt aaaagtgtgt ggatgtgatg
      961 aatacttcct agaaaaatat cctctgagtc agtataagta tataagaagc tgtataatgc
     1021 ttgggaggat gcccaatttg atgttgatgg ctaaagaaag cctttattct caactgccaa
     1081 tggactgttt tacaatgcca tcttattcca gacgcatttc cacagctaca ccatatatga
     1141 atggagaaac atctacaaaa tccctttggg ttataaatag tgcactcaga ataaaaattc
     1201 tttgtgcaac ctacgtgaat gtaaatattc gagacattga taagatctat gttcgaacag
     1261 gtatctacca tggaggagaa cccttatgtg acaatgtgaa cactcaaaga gtaccttgtt
     1321 ccaatcccag gtggaatgaa tggctgaatt atgatatata cattcctgat cttcctcgtg
     1381 ctgctcgact ttgcctttcc atttgctctg ttaaaggccg aaagggtgct aaagaggaac
     1441 actgtccatt ggcatgggga aatataaact tgtttgatta cacagacact ctagtatctg
     1501 gaaaaatggc tttgaatctt tggccagtac ctcatggatt agaagatttg ctgaacccta
     1561 ttggtgttac tggatcaaat ccaaataaag aaactccatg cttagagttg gagtttgact
     1621 ggttcagcag tgtggtaaag ttcccagata tgtcagtgat tgaagagcat gccaattggt
     1681 ctgtatcccg agaagcagga tttagctatt cccacgcagg actgagtaac agactagcta
     1741 gagacaatga attaagggaa aatgacaaag aacagctcaa agcaatttct acacgagatc
     1801 ctctctctga aatcactgag caggagaaag attttctatg gagtcacaga cactattgtg
     1861 taactatccc cgaaattcta cccaaattgc ttctgtctgt taaatggaat tctagagatg
     1921 aagtagccca gatgtattgc ttggtaaaag attggcctcc aatcaaacct gaacaggcta
     1981 tggaacttct ggactgtaat tacccagatc ctatggttcg aggttttgct gttcggtgct
     2041 tggaaaaata tttaacagat gacaaacttt ctcagtattt aattcagcta gtacaggtcc
     2101 taaaatatga acaatatttg gataacttgc ttgtgagatt tttactgaag aaagcattga
     2161 ctaatcaaag gattgggcac tttttctttt ggcatttaaa atctgagatg cacaataaaa
     2221 cagttagcca gaggtttggc ctgcttttgg agtcctattg tcgtgcatgt gggatgtatt
     2281 tgaagcacct gaataggcaa gtcgaggcaa tggaaaagct cattaactta actgacattc
     2341 tcaaacagga gaagaaggat gaaacacaaa aggtacagat gaagttttta gttgagcaaa
     2401 tgaggcgacc agatttcatg gatgctctac agggctttct gtctcctcta aaccctgctc
     2461 atcaactagg aaacctcagg cttgaagagt gtcgaattat gtcctctgca aaaaggccac
     2521 tgtggttgaa ttgggagaac ccagacatca tgtcagagtt actgtttcag aacaatgaga
     2581 tcatctttaa aaatggggat gatttacggc aagatatgct aacacttcaa attattcgta
     2641 ttatggaaaa tatctggcaa aatcaaggtc ttgatcttcg aatgttacct tatggttgtc
     2701 tgtcaatcgg tgactgtgtg ggacttattg aggtggtgcg aaattctcac actattatgc
     2761 aaattcagtg caaaggcggc ttgaaaggtg cactgcagtt caacagccac acactacatc
     2821 agtggctcaa agacaagaac aaaggagaaa tatatgatgc agccattgac ctgtttacac
     2881 gttcatgtgc tggatactgt gtagctacct tcattttggg aattggagat cgtcacaata
     2941 gtaacatcat ggtgaaagac gatggacaac tgtttcatat agattttgga cactttttgg
     3001 atcacaagaa gaaaaaattt ggttataaac gagaacgtgt gccatttgtt ttgacacagg
     3061 atttcttaat agtgattagt aaaggagccc aagaatgcac aaagacaaga gaatttgaga
     3121 ggtttcagga gatgtgttac aaggcttatc tagctattcg acagcatgcc aatctcttca
     3181 taaatctttt ctcaatgatg cttggctctg gaatgccaga actacaatct tttgatgaca
     3241 ttgcatacat tcgaaagacc ctagccttag ataaaactga gcaagaggct ttggagtatt
     3301 tcatgaaaca aatgaatgat gcacatcatg gtggctggac aacaaaaatg gattggatct
     3361 tccacacaat taaacagcat gcattgaact caactttctt gtacaaagtt ggcattatag
     3421 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     3481 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     3541 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     3601 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     3661 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     3721 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3781 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     3841 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     3901 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3961 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     4021 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     4081 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     4141 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     4201 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     4261 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     4321 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     4381 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4441 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4501 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4561 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     4621 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     4681 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4741 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4801 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4861 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4921 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4981 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5041 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5101 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     5161 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5221 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5281 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5341 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5401 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5461 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5521 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5581 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     5641 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     5701 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     5761 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     5821 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     5881 agttccctac tctcgcg
//
