LOCUS       sequence-125905-        4865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4865)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E172 Hs.PIK3R1-nostop
FEATURES             Location/Qualifiers
     source          1..4865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2356..2455)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2832..3623
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3716..4304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4205..4224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4458..4475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4634..4661)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4753..4839)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgag tgctgagggg taccagtaca gagcgctgta tgattataaa aaggaaagag
      241 aagaagatat tgacttgcac ttgggtgaca tattgactgt gaataaaggg tccttagtag
      301 ctcttggatt cagtgatgga caggaagcca ggcctgaaga aattggctgg ttaaatggct
      361 ataatgaaac cacaggggaa aggggggact ttccgggaac ttacgtagaa tatattggaa
      421 ggaaaaaaat ctcgcctccc acaccaaagc cccggccacc tcggcctctt cctgttgcac
      481 caggttcttc gaaaactgaa gcagatgttg aacaacaagc tttgactctc ccggatcttg
      541 cagagcagtt tgcccctcct gacattgccc cgcctcttct tatcaagctc gtggaagcca
      601 ttgaaaagaa aggtctggaa tgttcaactc tatacagaac acagagctcc agcaacctgg
      661 cagaattacg acagcttctt gattgtgata caccctccgt ggacttggaa atgatcgatg
      721 tgcacgtttt ggctgacgct ttcaaacgct atctcctgga cttaccaaat cctgtcattc
      781 cagcagccgt ttacagtgaa atgatttctt tagctccaga agtacaaagc tccgaagaat
      841 atattcagct attgaagaag cttattaggt cgcctagcat acctcatcag tattggctta
      901 cgcttcagta tttgttaaaa catttcttca agctctctca aacctccagc aaaaatctgt
      961 tgaatgcaag agtactctct gaaattttca gccctatgct tttcagattc tcagcagcca
     1021 gctctgataa tactgaaaac ctcataaaag ttatagaaat tttaatctca actgaatgga
     1081 atgaacgaca gcctgcacca gcactgcctc ctaaaccacc aaaacctact actgtagcca
     1141 acaacggtat gaataacaat atgtccttac aagatgctga atggtactgg ggagatatct
     1201 cgagggaaga agtgaatgaa aaacttcgag atacagcaga cgggaccttt ttggtacgag
     1261 atgcgtctac taaaatgcat ggtgattata ctcttacact aaggaaaggg ggaaataaca
     1321 aattaatcaa aatatttcat cgagatggga aatatggctt ctctgaccca ttaaccttca
     1381 gttctgtggt tgaattaata aaccactacc ggaatgaatc tctagctcag tataatccca
     1441 aattggatgt gaaattactt tatccagtat ccaaatacca acaggatcaa gttgtcaaag
     1501 aagataatat tgaagctgta gggaaaaaat tacatgaata taacactcag tttcaagaaa
     1561 aaagtcgaga atatgataga ttatatgaag aatatacccg cacatcccag gaaatccaaa
     1621 tgaaaaggac agctattgaa gcatttaatg aaaccataaa aatatttgaa gaacagtgcc
     1681 agacccaaga gcggtacagc aaagaataca tagaaaagtt taaacgtgaa ggcaatgaga
     1741 aagaaataca aaggattatg cataattatg ataagttgaa gtctcgaatc agtgaaatta
     1801 ttgacagtag aagaagattg gaagaagact tgaagaagca ggcagctgag tatcgagaaa
     1861 ttgacaaacg tatgaacagc attaaaccag accttatcca gctgagaaag acgagagacc
     1921 aatacttgat gtggttgact caaaaaggtg ttcggcaaaa gaagttgaac gagtggttgg
     1981 gcaatgaaaa cactgaagac caatattcac tggtggaaga tgatgaagat ttgccccatc
     2041 atgatgagaa gacatggaat gttggaagca gcaaccgaaa caaagctgaa aacctgttgc
     2101 gagggaagcg agatggcact tttcttgtcc gggagagcag taaacagggc tgctatgcct
     2161 gctctgtagt ggtggacggc gaagtaaagc attgtgtcat aaacaaaaca gcaactggct
     2221 atggctttgc cgagccctat aacttgtaca gctctctgaa agaactggtg ctacattacc
     2281 aacacacctc ccttgtgcag cacaacgact ccctcaatgt cacactagcc tacccagtat
     2341 atgcacagca gaggcgatca actttcttgt acaaagttgg cattatagga aagcattgct
     2401 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     2461 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2521 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2581 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2641 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2701 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2761 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2821 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2881 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2941 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3001 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     3061 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3121 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3181 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3241 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3301 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3361 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3421 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3481 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3541 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3601 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3661 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3721 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3781 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3841 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3901 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3961 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4021 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4081 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     4141 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4201 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4261 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4321 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4381 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4441 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4501 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4561 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     4621 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4681 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4741 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4801 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4861 tcgcg
//
