LOCUS       sequence-125924-        4373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PRKAA1 [NM_006251.5] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4373)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E191 Hs.PRKAA1
FEATURES             Location/Qualifiers
     source          1..4373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1864..1963)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2340..3131
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3224..3812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3713..3732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3966..3983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4142..4169)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4261..4347)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcg cagactcagt tcctggagaa agatggcgac agccgagaag cagaaacacg
      241 acgggcgggt gaagatcggc cactacattc tgggtgacac gctgggggtc ggcaccttcg
      301 gcaaagtgaa ggttggcaaa catgaattga ctgggcataa agtagctgtg aagatactca
      361 atcgacagaa gattcggagc cttgatgtgg taggaaaaat ccgcagagaa attcagaacc
      421 tcaagctttt caggcatcct catataatta aactgtacca ggtcatcagt acaccatctg
      481 atattttcat ggtgatggaa tatgtctcag gaggagagct atttgattat atctgtaaga
      541 atggaaggct ggatgaaaaa gaaagtcggc gtctgttcca acagatcctt tctggtgtgg
      601 attattgtca caggcatatg gtggtccata gagatttgaa acctgaaaat gtcctgcttg
      661 atgcacacat gaatgcaaag atagctgatt ttggtctttc aaacatgatg tcagatggtg
      721 aatttttaag aacaagttgt ggctcaccca actatgctgc accagaagta atttcaggaa
      781 gattgtatgc aggcccagag gtagatatat ggagcagtgg ggttattctc tatgctttat
      841 tatgtggaac ccttccattt gatgatgacc atgtgccaac tctttttaag aagatatgtg
      901 atgggatctt ctatacccct caatatttaa atccttctgt gattagcctt ttgaaacata
      961 tgctgcaggt ggatcccatg aagagggcca caatcaaaga tatcagggaa catgaatggt
     1021 ttaaacagga ccttccaaaa tatctctttc ctgaggatcc atcatatagt tcaaccatga
     1081 ttgatgatga agccttaaaa gaagtatgtg aaaagtttga gtgctcagaa gaggaagttc
     1141 tcagctgtct ttacaacaga aatcaccagg atcctttggc agttgcctac catctcataa
     1201 tagataacag gagaataatg aatgaagcca aagatttcta tttggcgaca agcccacctg
     1261 attcttttct tgatgatcat cacctgactc ggccccatcc tgaaagagta ccattcttgg
     1321 ttgctgaaac accaagggca cgccataccc ttgatgaatt aaatccacag aaatccaaac
     1381 accaaggtgt aaggaaagca aaatggcatt taggaattag aagtcaaagt cgaccaaatg
     1441 atattatggc agaagtatgt agagcaatca aacaattgga ttatgaatgg aaggttgtaa
     1501 acccatatta tttgcgtgta cgaaggaaga atcctgtgac aagcacttac tccaaaatga
     1561 gtctacagtt ataccaagtg gatagtagaa cttatctact ggatttccgt agtattgatg
     1621 atgaaattac agaagccaaa tcagggactg ctactccaca gagatcggga tcagttagca
     1681 actatcgatc ttgccaaagg agtgattcag atgctgaggc tcaaggaaaa tcctcagaag
     1741 tttctcttac ctcatctgtg acctcacttg actcttctcc tgttgaccta actccaagac
     1801 ctggaagtca cacaatagaa ttttttgaga tgtgtgcaaa tctaattaaa attcttgcac
     1861 aataatcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     1921 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     1981 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     2041 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     2101 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2161 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     2221 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2281 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     2341 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     2401 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     2461 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2521 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2581 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2641 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2701 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2761 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2821 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2881 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2941 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     3001 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     3061 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     3121 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     3181 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3241 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3301 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3361 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3421 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3481 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3541 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3601 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3661 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3721 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3781 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3841 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3901 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3961 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4021 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4081 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     4141 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4201 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4261 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4321 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
