LOCUS       sequence-126030-        4271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RPS6KB1 [NM_003161.3] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4271)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E297 Hs.RPS6KB1
FEATURES             Location/Qualifiers
     source          1..4271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1762..1861)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2238..3029
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3122..3710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3611..3630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3864..3881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4040..4067)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4159..4245)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgag gcgacgaagg aggcgggacg gcttttaccc agccccggac ttccgggaca
      241 gggaagctga ggacatggca ggagtgtttg acatagacct ggaccagcca gaggacgcgg
      301 gctctgagga tgagctggag gaggggggtc agttaaatga aagcatggac catgggggag
      361 ttggaccata tgaacttggc atggaacatt gtgagaaatt tgaaatctca gaaactagtg
      421 tgaacagagg gccagaaaaa atcagaccag aatgttttga gctacttcgg gtacttggta
      481 aagggggcta tggaaaggtt tttcaagtac gaaaagtaac aggagcaaat actgggaaaa
      541 tatttgccat gaaggtgctt aaaaaggcaa tgatagtaag aaatgctaaa gatacagctc
      601 atacaaaagc agaacggaat attctggagg aagtaaagca tcccttcatc gtggatttaa
      661 tttatgcctt tcagactggt ggaaaactct acctcatcct tgagtatctc agtggaggag
      721 aactatttat gcagttagaa agagagggaa tatttatgga agacactgcc tgcttttact
      781 tggcagaaat ctccatggct ttggggcatt tacatcaaaa ggggatcatc tacagagacc
      841 tgaagccgga gaatatcatg cttaatcacc aaggtcatgt gaaactaaca gactttggac
      901 tatgcaaaga atctattcat gatggaacag tcacacacac attttgtgga acaatagagt
      961 acatggcccc tgaaatcttg atgagaagtg gccacaatcg tgctgtggat tggtggagtt
     1021 tgggagcatt aatgtatgac atgctgactg gagcaccccc attcactggg gagaatagaa
     1081 agaaaacaat tgacaaaatc ctcaaatgta aactcaattt gcctccctac ctcacacaag
     1141 aagccagaga tctgcttaaa aagctgctga aaagaaatgc tgcttctcgt ctgggagctg
     1201 gtcctgggga cgctggagaa gttcaagctc atccattctt tagacacatt aactgggaag
     1261 aacttctggc tcgaaaggtg gagcccccct ttaaacctct gttgcaatct gaagaggatg
     1321 taagtcagtt tgattccaag tttacacgtc agacacctgt cgacagccca gatgactcaa
     1381 ctctcagtga aagtgccaat caggtctttc tgggttttac atatgtggct ccatctgtac
     1441 ttgaaagtgt gaaagaaaag ttttcctttg aaccaaaaat ccgatcacct cgaagattta
     1501 ttggcagccc acgaacacct gtcagcccag tcaaattttc tcctggggat ttctggggaa
     1561 gaggtgcttc ggccagcaca gcaaatcctc agacacctgt ggaataccca atggaaacaa
     1621 gtggcataga gcagatggat gtgacaatga gtggggaagc atcggcacca cttccaatac
     1681 gacagccgaa ctctgggcca tacaaaaaac aagcttttcc catgatctcc aaacggccag
     1741 agcacctgcg tatgaatcta taatcaactt tcttgtacaa agttggcatt ataggaaagc
     1801 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1861 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1921 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1981 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2041 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2101 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2161 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2221 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2281 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2341 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2401 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2461 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2521 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2581 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2641 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2701 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2761 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2821 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2881 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2941 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3001 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3061 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3121 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3181 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3241 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3301 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3361 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3481 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     3541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3661 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3781 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3841 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3901 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3961 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4021 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4081 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4141 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4201 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     4261 ctactctcgc g
//
