LOCUS       sequence-126031-        4268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RPS6KB1 [NM_003161.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E298 Hs.RPS6KB1-nostop
FEATURES             Location/Qualifiers
     source          1..4268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1759..1858)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2235..3026
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3119..3707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3608..3627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3861..3878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4037..4064)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4156..4242)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgag gcgacgaagg aggcgggacg gcttttaccc agccccggac ttccgggaca
      241 gggaagctga ggacatggca ggagtgtttg acatagacct ggaccagcca gaggacgcgg
      301 gctctgagga tgagctggag gaggggggtc agttaaatga aagcatggac catgggggag
      361 ttggaccata tgaacttggc atggaacatt gtgagaaatt tgaaatctca gaaactagtg
      421 tgaacagagg gccagaaaaa atcagaccag aatgttttga gctacttcgg gtacttggta
      481 aagggggcta tggaaaggtt tttcaagtac gaaaagtaac aggagcaaat actgggaaaa
      541 tatttgccat gaaggtgctt aaaaaggcaa tgatagtaag aaatgctaaa gatacagctc
      601 atacaaaagc agaacggaat attctggagg aagtaaagca tcccttcatc gtggatttaa
      661 tttatgcctt tcagactggt ggaaaactct acctcatcct tgagtatctc agtggaggag
      721 aactatttat gcagttagaa agagagggaa tatttatgga agacactgcc tgcttttact
      781 tggcagaaat ctccatggct ttggggcatt tacatcaaaa ggggatcatc tacagagacc
      841 tgaagccgga gaatatcatg cttaatcacc aaggtcatgt gaaactaaca gactttggac
      901 tatgcaaaga atctattcat gatggaacag tcacacacac attttgtgga acaatagagt
      961 acatggcccc tgaaatcttg atgagaagtg gccacaatcg tgctgtggat tggtggagtt
     1021 tgggagcatt aatgtatgac atgctgactg gagcaccccc attcactggg gagaatagaa
     1081 agaaaacaat tgacaaaatc ctcaaatgta aactcaattt gcctccctac ctcacacaag
     1141 aagccagaga tctgcttaaa aagctgctga aaagaaatgc tgcttctcgt ctgggagctg
     1201 gtcctgggga cgctggagaa gttcaagctc atccattctt tagacacatt aactgggaag
     1261 aacttctggc tcgaaaggtg gagcccccct ttaaacctct gttgcaatct gaagaggatg
     1321 taagtcagtt tgattccaag tttacacgtc agacacctgt cgacagccca gatgactcaa
     1381 ctctcagtga aagtgccaat caggtctttc tgggttttac atatgtggct ccatctgtac
     1441 ttgaaagtgt gaaagaaaag ttttcctttg aaccaaaaat ccgatcacct cgaagattta
     1501 ttggcagccc acgaacacct gtcagcccag tcaaattttc tcctggggat ttctggggaa
     1561 gaggtgcttc ggccagcaca gcaaatcctc agacacctgt ggaataccca atggaaacaa
     1621 gtggcataga gcagatggat gtgacaatga gtggggaagc atcggcacca cttccaatac
     1681 gacagccgaa ctctgggcca tacaaaaaac aagcttttcc catgatctcc aaacggccag
     1741 agcacctgcg tatgaatcta tcaactttct tgtacaaagt tggcattata ggaaagcatt
     1801 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     1861 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1921 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1981 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2041 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2101 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2161 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2221 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2281 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2341 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2401 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2461 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2521 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2581 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2641 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2701 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2761 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2821 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2881 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2941 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3001 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3061 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3241 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3481 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3901 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3961 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4021 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4081 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4141 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4201 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4261 ctctcgcg
//
