LOCUS       sequence-126036-        3845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human SAV1 [NM_021818.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3845)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E303 Hs.SAV1
FEATURES             Location/Qualifiers
     source          1..3845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1336..1435)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1812..2603
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2696..3284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3185..3204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3438..3455
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3614..3641)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3733..3819)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgct gtcccgaaag aaaaccaaaa acgaagtgtc caagccggcc gaggtgcagg
      241 ggaagtacgt gaagaaggag acgtcgcctc tgcttcggaa tcttatgcct tcattcatcc
      301 ggcatggtcc aacaattcca agacgaactg atatctgtct tccagattca agccctaatg
      361 ccttttcaac ttctggagat gtagtttcaa gaaaccagag tttccttaga actccaattc
      421 aaagaacacc tcatgaaata atgagaagag aaagcaacag attatctgca ccttcttatc
      481 ttgccagaag tctagcagat gtccctagag agtatggttc ttctcagtca tttgtaacgg
      541 aagttagttt tgctgttgaa aatggagact ctggttcccg atattattat tcagacaatt
      601 tttttgatgg tcagagaaag cggccacttg gagatcgtgc acatgaagac tacagatatt
      661 atgaatacaa ccatgatctc ttccaaagaa tgccacagaa tcaggggagg catgcttcag
      721 gtattgggag agttgctgct acatctttag gaaatttgac taaccatggt tctgaagatt
      781 taccccttcc tcctggctgg tctgtggact ggacaatgag agggagaaaa tattatatag
      841 atcataacac aaatacaact cactggagcc atcctcttga gcgagaagga cttcctcctg
      901 gatgggaacg agttgagtca tccgaatttg gaacctatta tgtagatcac acaaataaga
      961 aggcccaata caggcatccc tgtgctccta gtgtacctcg gtatgatcaa ccacctcctg
     1021 tcacatacca gccacagcaa actgaaagaa atcagtccct tctggtacct gcaaatccat
     1081 atcatactgc agaaattcct gactggcttc aggtttacgc acgagcccct gtgaaatatg
     1141 accacattct gaagtgggaa ctcttccagc tggctgacct ggatacatac cagggaatgc
     1201 taaagttgct cttcatgaaa gaattggagc agattgttaa aatgtatgaa gcatacagac
     1261 aagcccttct tacagagttg gaaaaccgaa agcagagaca gcagtggtat gcccaacaac
     1321 atggaaaaaa tttttaatca actttcttgt acaaagttgg cattatagga aagcattgct
     1381 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgcatgc
     1441 aagcttagat cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     1501 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     1561 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     1621 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     1681 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     1741 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     1801 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1861 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1921 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     1981 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2041 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2101 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2161 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2221 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2281 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2341 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2401 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2461 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2521 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     2581 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     2641 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2701 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2761 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2821 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2881 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2941 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3001 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3061 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     3121 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3181 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3241 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3301 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3361 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3421 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3481 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3541 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     3601 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3661 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     3721 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     3781 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     3841 tcgcg
//
