LOCUS       sequence-126075-        4880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human TBK1 [NM_013254.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4880)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E342 Hs.TBK1-nostop
FEATURES             Location/Qualifiers
     source          1..4880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2371..2470)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2847..3638
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3731..4319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4220..4239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4473..4490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4649..4676)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4768..4854)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgca gagcacttct aatcatctgt ggcttttatc tgatatttta ggccaaggag
      241 ctactgcaaa tgtctttcgt ggaagacata agaaaactgg tgatttattt gctatcaaag
      301 tatttaataa cataagcttc cttcgtccag tggatgttca aatgagagaa tttgaagtgt
      361 tgaaaaaact caatcacaaa aatattgtca aattatttgc tattgaagag gagacaacaa
      421 caagacataa agtacttatt atggaatttt gtccatgtgg gagtttatac actgttttag
      481 aagaaccttc taatgcctat ggactaccag aatctgaatt cttaattgtt ttgcgagatg
      541 tggtgggtgg aatgaatcat ctacgagaga atggtatagt gcaccgtgat atcaagccag
      601 gaaatatcat gcgtgttata ggggaagatg gacagtctgt gtacaaactc acagattttg
      661 gtgcagctag agaattagaa gatgatgagc agtttgtttc tctgtatggc acagaagaat
      721 atttgcaccc tgatatgtat gagagagcag tgctaagaaa agatcatcag aagaaatatg
      781 gagcaacagt tgatctttgg agcattgggg taacatttta ccatgcagct actggatcac
      841 tgccatttag accctttgaa gggcctcgta ggaataaaga agtgatgtat aaaataatta
      901 caggaaagcc ttctggtgca atatctggag tacagaaagc agaaaatgga ccaattgact
      961 ggagtggaga catgcctgtt tcttgcagtc tttctcgggg tcttcaggtt ctacttaccc
     1021 ctgttcttgc aaacatcctt gaagcagatc aggaaaagtg ttggggtttt gaccagtttt
     1081 ttgcagaaac tagtgatata cttcaccgaa tggtaattca tgttttttcg ctacaacaaa
     1141 tgacagctca taagatttat atacatagct ataatactgc tactatattt catgaactgg
     1201 tatataaaca aaccaaaatt atttcttcaa atcaagaact tatctacgaa gggcgacgct
     1261 tagtcttaga acctggaagg ctggcacaac atttccctaa aactactgag gaaaacccta
     1321 tatttgtagt aagccgggaa cctctgaata ccataggatt aatatatgaa aaaatttccc
     1381 tccctaaagt acatccacgt tatgatttag acggggatgc tagcatggct aaggcaataa
     1441 caggggttgt gtgttatgcc tgcagaattg ccagtacctt actgctttat caggaattaa
     1501 tgcgaaaggg gatacgatgg ctgattgaat taattaaaga tgattacaat gaaactgttc
     1561 acaaaaagac agaagttgtg atcacattgg atttctgtat cagaaacatt gaaaaaactg
     1621 tgaaagtata tgaaaagttg atgaagatca acctggaagc ggcagagtta ggtgaaattt
     1681 cagacataca caccaaattg ttgagacttt ccagttctca gggaacaata gaaaccagtc
     1741 ttcaggatat cgacagcaga ttatctccag gtggatcact ggcagacgca tgggcacatc
     1801 aagaaggcac tcatccgaaa gacagaaatg tagaaaaact acaagtcctg ttaaattgca
     1861 tgacagagat ttactatcag ttcaaaaaag acaaagcaga acgtagatta gcttataatg
     1921 aagaacaaat ccacaaattt gataagcaaa aactgtatta ccatgccaca aaagctatga
     1981 cgcactttac agatgaatgt gttaaaaagt atgaggcatt tttgaataag tcagaagaat
     2041 ggataagaaa gatgcttcat cttaggaaac agttattatc gctgactaat cagtgttttg
     2101 atattgaaga agaagtatca aaatatcaag aatatactaa tgagttacaa gaaactctgc
     2161 ctcagaaaat gtttacagct tccagtggaa tcaaacatac catgacccca atttatccaa
     2221 gttctaacac attagtagaa atgactcttg gtatgaagaa attaaaggaa gagatggaag
     2281 gggtggttaa agaacttgct gaaaataacc acattttaga aaggtttggc tctttaacca
     2341 tggatggtgg ccttcgcaac gttgactgtc tttcaacttt cttgtacaaa gttggcatta
     2401 taggaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2461 tcattatttg catgcaagct tagatcctct agaccagcca ggacagaaat gcctcgactt
     2521 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2581 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2641 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2701 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2761 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2821 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2881 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2941 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     3001 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     3061 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     3121 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     3181 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3241 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3301 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3361 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3421 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3481 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3541 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3601 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3661 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3841 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     4141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4381 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4441 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4501 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4561 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4621 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4681 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4741 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4801 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4861 ggcagttccc tactctcgcg
//
